Researcher Profile

Researcher Profile

Edward P. O'Brien, Jr., PhD

Edward P. O'Brien, Jr., PhD

Assistant Professor, Chemistry
Scientific Program:Mechanisms of Carcinogenesis
Disease Teams:
Institute for CyberScience (ICS) - Co-hire

Research Interests

  • Proteins
  • Ribosomes
  • Protein Folding
  • Kinetics
  • Codon
  • Peptides
  • Molecular Models
  • Messenger RNA
  • Amino Acids
  • Proteome
  • Temperature
  • Static Electricity

Recent Publications


Nguyen, HL, Lan, PD, Thai, NQ, Nissley, DA, O'Brien, EP & Li, MS 2020, 'Does SARS-CoV-2 bind to human ACE2 more strongly than does SARS-CoV?', Journal of Physical Chemistry B, vol. 124, no. 34, pp. 7336-7347.
Nissley, DA, Vu, QV, Trovato, F, Ahmed, N, Jiang, Y, Li, MS & O'Brien, EP 2020, 'Electrostatic Interactions Govern Extreme Nascent Protein Ejection Times from Ribosomes and Can Delay Ribosome Recycling', Journal of the American Chemical Society, vol. 142, no. 13, pp. 6103-6110.
Leininger, SE, Deutsch, C & O’Brien, EP 2020, 'Forcing the ribosome to change its message', Journal of Biological Chemistry, vol. 295, no. 20, pp. 6809-6810.
Wolfe, DK, Persichetti, JR, Sharma, AK, Hudson, PS, Woodcock, HL & O'brien, EP 2020, 'Hierarchical Markov State Model Building to Describe Molecular Processes', Journal of Chemical Theory and Computation, vol. 16, no. 3, pp. 1816-1826.
Ahmed, N, Friedrich, UA, Sormanni, P, Ciryam, P, Altman, NS, Bukau, B, Kramer, G & O'Brien, EP 2020, 'Pairs of amino acids at the P- and A-sites of the ribosome predictably and causally modulate translation-elongation rates: Amino acid pairs module translation-elongation rates', Journal of Molecular Biology, vol. 432, no. 24, 166696.


Sharma, AK, Sormanni, P, Ahmed, N, Ciryam, P, Friedrich, UA, Kramer, G & O'Brien, EP 2019, 'A chemical kinetic basis for measuring translation initiation and elongation rates from ribosome profiling data', PLoS computational biology, vol. 15, no. 5, e1007070.
Leininger, SE, Trovato, F, Nissley, DA & O’Brien, EP 2019, 'Domain topology, stability, and translation speed determine mechanical force generation on the ribosome', Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 12, pp. 5523-5532.
Ahmed, N, Sormanni, P, Ciryam, P, Vendruscolo, M, Dobson, CM & O’Brien, EP 2019, 'Identifying A- and P-site locations on ribosome-protected mRNA fragments using Integer Programming', Scientific reports, vol. 9, no. 1, 6256.
Leininger, SE, Narayan, K, Deutsch, C & O'Brien, EP 2019, 'Mechanochemistry in Translation', Biochemistry, vol. 58, no. 47, pp. 4657-4666.


Sharma, AK, Ahmed, N & O'Brien, EP 2018, 'Determinants of translation speed are randomly distributed across transcripts resulting in a universal scaling of protein synthesis times', Physical Review E, vol. 97, no. 2, 022409.
Nguyen, HL, Pham, DL, O'Brien, EP & Li, MS 2018, 'Erythromycin leads to differential protein expression through differences in electrostatic and dispersion interactions with nascent proteins', Scientific reports, vol. 8, no. 1, 6460.
Samelson, AJ, Bolin, E, Costello, SM, Sharma, AK, O’Brien, EP & Marqusee, S 2018, 'Kinetic and structural comparison of a protein’s cotranslational folding and refolding pathways', Science Advances, vol. 4, no. 5, eaas9098.
Sharma, AK & O'Brien, EP 2018, 'Non-equilibrium coupling of protein structure and function to translation–elongation kinetics', Current Opinion in Structural Biology, vol. 49, pp. 94-103.
Fritch, B, Kosolapov, A, Hudson, P, Nissley, DA, Woodcock, HL, Deutsch, C & O'Brien, EP 2018, 'Origins of the Mechanochemical Coupling of Peptide Bond Formation to Protein Synthesis', Journal of the American Chemical Society, vol. 140, no. 15, pp. 5077-5087.
Nissley, DA & O'Brien, EP 2018, 'Structural Origins of FRET-Observed Nascent Chain Compaction on the Ribosome', Journal of Physical Chemistry B, vol. 122, no. 43, pp. 9927-9937.


Trovato, F & O'Brien, EP 2017, 'Fast Protein Translation Can Promote Co- and Posttranslational Folding of Misfolding-Prone Proteins', Biophysical journal, vol. 112, no. 9, pp. 1807-1819.
Sharma, AK & O'Brien, EP 2017, 'Increasing Protein Production Rates Can Decrease the Rate at Which Functional Protein is Produced and Their Steady-State Levels', Journal of Physical Chemistry B, vol. 121, no. 28, pp. 6775-6784.
Döring, K, Ahmed, N, Riemer, T, Suresh, HG, Vainshtein, Y, Habich, M, Riemer, J, Mayer, MP, O'Brien, EP, Kramer, G & Bukau, B 2017, 'Profiling Ssb-Nascent Chain Interactions Reveals Principles of Hsp70-Assisted Folding', Cell, vol. 170, no. 2, pp. 298-311.e20.

Clinical Trials Search

Children (age < 18 years)
Adults (age >= 18 years)