Researcher Profile

Researcher Profile

Nikolay Dokholyan, PhD, MS

Nikolay Dokholyan, PhD, MS

G. Thomas Passananti Professor and Vice Chair for Research, Department of Pharmacology
Professor, Department of Biochemistry and Molecular Biology
Penn State Neuroscience Institute
Scientific Program:Next-Generation Therapies
nxd338@psu.edu

Research Interests

The mission of Dr. Nikolay Dokholyan's laboratory is to develop and apply integrated computational and experimental strategies to understand, sense and control misfolded proteins in order to uncover the etiologies of human neurodegenerative diseases and develop therapeutics to fight them.

The lab aims to understand the molecular disease mechanisms of ALS: How does the misfolding of superoxide dismutase (SOD1) lead to the formation of toxic oligomeric intermediates? Using biochemical and biophysical approaches and innovative computation, the Dokholyan lab determined putative structures of SOD1 oligomers and is currently elucidating the downstream pathways that lead to motorneuron death. Structures of toxic oligomers provide targets for drug discovery, which the lab is pursuing.

Neurodegenerative diseases such as ALS, Alzheimer’s, Huntington’s, Parkinson’s and prion diseases share similar processes associated with protein misfolding and aggregation. These similarities suggest common pathways leading to neuron death that eventually result in a disease. The lab is working toward understanding the general principles of protein misfolding in neurodegenerative diseases through computational and experimental approaches.

To sense and control protein conformations, the lab is working toward development of genetically-encoded proteins that bind and report rare/intermediate conformations of target molecules or alter their state using drugs or light.

One of the critical components of the lab's integrative research is drug discovery, focusing on both biological therapeutics and small molecule screening. The lab developed a fully flexible docking algorithm, MedusaDock, that allows for virtual screening of compounds and is is an important asset for small molecule drug discovery efforts.

The lab has developed novel approaches to molecular dynamics simulations and modeling, allowing studies of biological molecules at time scales relevant to biological systems. These approaches synergistically integrate rapid dynamics simulations, molecular modeling and design, and biochemical and cellular biology experiments, allowing for significant strides in understanding the etiology of misfolding diseases.

  • Proteins
  • Molecular Dynamics Simulation
  • Mutation
  • RNA
  • Ligands
  • Superoxide Dismutase-1
  • Thermodynamics
  • Amyotrophic Lateral Sclerosis
  • Protein Folding
  • Ryanodine Receptor Calcium Release Channel
  • Peptides
  • Cystic Fibrosis Transmembrane Conductance Regulator

Recent Publications

2021

Chen, YC, Dinavahi, SS, Feng, Q, Gowda, R, Ramisetti, S, Xia, X, LaPenna, KB, Chirasani, VR, Cho, SH, Hafenstein, SL, Battu, MB, Berg, A, Sharma, AK, Kirchhausen, T, Dokholyan, NV, Amin, S, He, P & Robertson, GP 2021, 'Activating Sphingosine-1-phospahte signaling in endothelial cells increases myosin light chain phosphorylation to decrease endothelial permeability thereby inhibiting cancer metastasis', Cancer Letters, vol. 506, pp. 107-119. https://doi.org/10.1016/j.canlet.2021.01.004
Nguyen, PH, Ramamoorthy, A, Sahoo, BR, Zheng, J, Faller, P, Straub, JE, Dominguez, L, Shea, JE, Dokholyan, NV, de Simone, A, Ma, B, Nussinov, R, Najafi, S, Ngo, ST, Loquet, A, Chiricotto, M, Ganguly, P, McCarty, J, Li, MS, Hall, C, Wang, Y, Miller, Y, Melchionna, S, Habenstein, B, Timr, S, Chen, J, Hnath, B, Strodel, B, Kayed, R, Lesné, S, Wei, G, Sterpone, F, Doig, AJ & Derreumaux, P 2021, 'Amyloid oligomers: A joint experimental/computational perspective on Alzheimer's disease, Parkinson's disease, type II diabetes, and amyotrophic lateral sclerosis', Chemical Reviews, vol. 121, no. 4, pp. 2545-2647. https://doi.org/10.1021/acs.chemrev.0c01122
Christy, TW, Giannetti, CA, Houlihan, G, Smola, MJ, Rice, GM, Wang, J, Dokholyan, NV, Laederach, A, Holliger, P & Weeks, KM 2021, 'Direct Mapping of Higher-Order RNA Interactions by SHAPE-JuMP', Biochemistry, vol. 60, no. 25, pp. 1971-1982. https://doi.org/10.1021/acs.biochem.1c00270
Vishweshwaraiah, YL, Chen, J & Dokholyan, NV 2021, 'Engineering an allosteric control of protein function', Journal of Physical Chemistry B, vol. 125, no. 7, pp. 1806-1814. https://doi.org/10.1021/acs.jpcb.0c11640
Fan, M, Wang, J, Jiang, H, Feng, Y, Mahdavi, M, Madduri, K, Kandemir, MT & Dokholyan, NV 2021, 'GPU-Accelerated Flexible Molecular Docking', Journal of Physical Chemistry B, vol. 125, no. 4, pp. 1049-1060. https://doi.org/10.1021/acs.jpcb.0c09051
Muralidharan, A, Samoshkin, A, Convertino, M, Piltonen, MH, Gris, P, Wang, J, Jiang, C, Klares, R, Linton, A, Ji, RR, Maixner, W, Dokholyan, NV, Mogil, JS & Diatchenko, L 2021, 'Identification and characterization of novel candidate compounds targeting 6- and 7-transmembrane μ-opioid receptor isoforms', British Journal of Pharmacology, vol. 178, no. 13, pp. 2709-2726. https://doi.org/10.1111/bph.15463
Li, J, Alam, M, Sha, CM, Wang, J, Dokholyan, N & Ghosh, S 2021, Invited: Drug Discovery Approaches using Quantum Machine Learning. in 2021 58th ACM/IEEE Design Automation Conference, DAC 2021. Proceedings - Design Automation Conference, vol. 2021-December, Institute of Electrical and Electronics Engineers Inc., pp. 1356-1359, 58th ACM/IEEE Design Automation Conference, DAC 2021, San Francisco, United States, 12/5/21. https://doi.org/10.1109/DAC18074.2021.9586268
Chirasani, VR, Popov, KI, Meissner, G & Dokholyan, NV 2022, 'Mapping co-regulatory interactions among ligand-binding sites in ryanodine receptor 1', Proteins: Structure, Function and Bioinformatics, vol. 90, no. 2, pp. 385-394. https://doi.org/10.1002/prot.26228
Zhovmer, AS, Manning, A, Smith, C, Hayes, JB, Burnette, DT, Wang, J, Cartagena-Rivera, AX, Dokholyan, NV, Singh, RK & Tabdanov, ED 2021, 'Mechanical Counterbalance of Kinesin and Dynein Motors in a Microtubular Network Regulates Cell Mechanics, 3D Architecture, and Mechanosensing', ACS nano, vol. 15, no. 11, pp. 17528-17548. https://doi.org/10.1021/acsnano.1c04435
Landoni, E, Fuca, G, Wang, J, Chirasani, VR, Yao, Z, Dukhovlinova, E, Ferrone, S, Savoldo, B, Hong, LK, Shou, P, Musio, S, Padelli, F, Finocchiaro, G, Droste, M, Kuhlman, B, Shamshiev, A, Pellegatta, S, Dokholyan, NV & Dotti, G 2021, 'Modifications to the framework regions eliminate chimeric antigen receptor tonic signaling', Cancer Immunology Research, vol. 9, no. 4, pp. 441-453. https://doi.org/10.1158/2326-6066.CIR-20-0451
Dokholyan, NV 2021, 'Nanoscale programming of cellular and physiological phenotypes: inorganic meets organic programming', NPJ systems biology and applications, vol. 7, no. 1, 15. https://doi.org/10.1038/s41540-021-00176-8
Choi, ES & Dokholyan, NV 2021, 'SOD1 oligomers in amyotrophic lateral sclerosis', Current Opinion in Structural Biology, vol. 66, pp. 225-230. https://doi.org/10.1016/j.sbi.2020.12.002
Serpa, JJ, Popov, KI, Petrotchenko, EV, Dokholyan, NV & Borchers, CH 2021, 'Structure of prion β-oligomers as determined by short-distance crosslinking constraint-guided discrete molecular dynamics simulations', Proteomics, vol. 21, no. 21-22, 2000298. https://doi.org/10.1002/pmic.202000298
Chen, J, Zaer, S, Drori, P, Zamel, J, Joron, K, Kalisman, N, Lerner, E & Dokholyan, NV 2021, 'The structural heterogeneity of α-synuclein is governed by several distinct subpopulations with interconversion times slower than milliseconds', Structure, vol. 29, no. 9, pp. 1048-1064.e6. https://doi.org/10.1016/j.str.2021.05.002
Reilley, DJ, Wang, J, Dokholyan, NV & Alexandrova, AN 2021, 'Titr-DMD - A rapid, coarse-grained Quasi-All-Atom constant pH molecular dynamics framework', Journal of Chemical Theory and Computation, vol. 17, no. 7, pp. 4538-4549. https://doi.org/10.1021/acs.jctc.1c00338
Vishweshwaraiah, YL, Chen, J, Chirasani, VR, Tabdanov, ED & Dokholyan, NV 2021, 'Two-input protein logic gate for computation in living cells', Nature communications, vol. 12, no. 1, 6615. https://doi.org/10.1038/s41467-021-26937-x

2020

Tyagi, A, Chandrasekaran, B, Kolluru, V, Baby, BV, Sripathi, CA, Ankem, MK, Ramisetti, SR, Chirasani, VR, Dokholyan, NV, Sharma, AK & Damodaran, C 2020, 'ASR490, a small molecule, overrides aberrant expression of notch1 in colorectal cancer', Molecular cancer therapeutics, vol. 19, no. 12, pp. 2422-2431. https://doi.org/10.1158/1535-7163.MCT-19-0949
Piltonen, M, Krokhotin, A, Parisien, M, Bérubé, P, Djambazian, H, Sladek, R, Dokholyan, NV, Shabalina, SA & Diatchenko, L 2021, 'Alternative Splicing of Opioid Receptor Genes Shows a Conserved Pattern for 6TM Receptor Variants', Cellular and Molecular Neurobiology, vol. 41, no. 5, pp. 1039-1055. https://doi.org/10.1007/s10571-020-00971-7
Piltonen, M, Krokhotin, A, Parisien, M, Bérubé, P, Djambazian, H, Sladek, R, Dokholyan, NV, Shabalina, SA & Diatchenko, L 2021, 'Correction to: Alternative Splicing of Opioid Receptor Genes Shows a Conserved Pattern for 6TM Receptor Variants (Cellular and Molecular Neurobiology, (2021), 41, 5, (1039-1055), 10.1007/s10571-020-00971-7)', Cellular and Molecular Neurobiology, vol. 41, no. 5, pp. 1057. https://doi.org/10.1007/s10571-020-00999-9
Dinavahi, SS, Gowda, R, Gowda, K, Bazewicz, CG, Chirasani, VR, Battu, MB, Berg, A, Dokholyan, NV, Amin, S & Robertson, GP 2020, 'Development of a Novel Multi-Isoform ALDH Inhibitor Effective as an Antimelanoma Agent', Molecular cancer therapeutics, vol. 19, no. 2, pp. 447-459. https://doi.org/10.1158/1535-7163.MCT-19-0360
Dokholyan, NV 2020, 'Experimentally-driven protein structure modeling', Journal of Proteomics, vol. 220, 103777. https://doi.org/10.1016/j.jprot.2020.103777
Jiang, H, Fan, M, Wang, J, Sarma, A, Mohanty, S, Dokholyan, NV, Mahdavi, M & Kandemir, MT 2020, 'Guiding conventional protein-ligand docking software with convolutional neural networks', Journal of Chemical Information and Modeling, vol. 60, no. 10, pp. 4594-4602. https://doi.org/10.1021/acs.jcim.0c00542
Makepeace, KAT, Brodie, NI, Popov, KI, Gudavicius, G, Nelson, CJ, Petrotchenko, EV, Dokholyan, NV & Borchers, CH 2020, 'Ligand-induced disorder-to-order transitions characterized by structural proteomics and molecular dynamics simulations', Journal of Proteomics, vol. 211, 103544. https://doi.org/10.1016/j.jprot.2019.103544
Wang, J, Jain, A, McDonald, LR, Gambogi, C, Lee, AL & Dokholyan, NV 2020, 'Mapping allosteric communications within individual proteins', Nature communications, vol. 11, no. 1, 3862. https://doi.org/10.1038/s41467-020-17618-2
Zhang, DY, Wang, J & Dokholyan, NV 2021, 'Prefusion spike protein stabilization through computational mutagenesis', Proteins: Structure, Function and Bioinformatics, vol. 89, no. 4, pp. 399-408. https://doi.org/10.1002/prot.26025
Miao, Z, Adamiak, RW, Antczak, M, Boniecki, MJ, Bujnicki, J, Chen, SJ, Cheng, CY, Cheng, Y, Chou, FC, Das, R, Dokholyan, NV, Ding, F, Geniesse, C, Jiang, Y, Joshi, A, Krokhotin, A, Magnus, M, Mailhot, O, Major, F, Mann, TH, Piątkowski, P, Pluta, R, Popenda, M, Sarzynska, J, Sun, L, Szachniuk, M, Tian, S, Wang, J, Wang, J, Watkins, AM, Wiedemann, J, Xiao, Y, Xu, X, Yesselman, JD, Zhang, D, Zhang, Y, Zhang, Z, Zhao, C, Zhao, P, Zhou, Y, Zok, T, Żyła, A, Ren, A, Batey, RT, Golden, BL, Huang, L, Lilley, DM, Liu, Y, Patel, DJ & Westhof, E 2020, 'RNA-Puzzles round IV: 3D Structure predictions of four ribozymes and two aptamers', RNA, vol. 26, no. 8, pp. 982-995. https://doi.org/10.1261/RNA.075341.120
Wu, HD, Kikuchi, M, Dagliyan, O, Aragaki, AK, Nakamura, H, Dokholyan, NV, Umehara, T & Inoue, T 2020, 'Rational design and implementation of a chemically inducible heterotrimerization system', Nature methods, vol. 17, no. 9, pp. 928-936. https://doi.org/10.1038/s41592-020-0913-x
Xu, L, Harms, FL, Chirasani, VR, Pasek, DA, Kortüm, F, Meinecke, P, Dokholyan, NV, Kutsche, K & Meissner, G 2020, 'Single-channel properties of skeletal muscle ryanodine receptor pore Δ4923FF4924 in two brothers with a lethal form of fetal akinesia', Cell Calcium, vol. 87, 102182. https://doi.org/10.1016/j.ceca.2020.102182
Ono, C, Fukuhara, T, Li, S, Wang, J, Sato, A, Izumi, T, Fauzyah, Y, Yamamoto, T, Morioka, Y, Dokholyan, NV, Standley, DM & Matsuura, Y 2020, 'Various miRNAs compensate the role of miR-122 on HCV replication', PLoS pathogens, vol. 16, no. 6 June, e1008308. https://doi.org/10.1371/journal.ppat.1008308

2019

Chirasani, VR, Xu, L, Addis, HG, Pasek, DA, Dokholyan, NV, Meissner, G & Yamaguchi, N 2019, 'A central core disease mutation in the Ca2+-binding site of skeletal muscle ryanodine receptor impairs single-channel regulation', American Journal of Physiology - Cell Physiology, vol. 317, no. 2, pp. C358-C365. https://doi.org/10.1152/ajpcell.00052.2019
Wodak, SJ, Paci, E, Dokholyan, NV, Berezovsky, IN, Horovitz, A, Li, J, Hilser, VJ, Bahar, I, Karanicolas, J, Stock, G, Hamm, P, Stote, RH, Eberhardt, J, Chebaro, Y, Dejaegere, A, Cecchini, M, Changeux, JP, Bolhuis, PG, Vreede, J, Faccioli, P, Orioli, S, Ravasio, R, Yan, L, Brito, C, Wyart, M, Gkeka, P, Rivalta, I, Palermo, G, McCammon, JA, Panecka-Hofman, J, Wade, RC, Di Pizio, A, Niv, MY, Nussinov, R, Tsai, CJ, Jang, H, Padhorny, D, Kozakov, D & McLeish, T 2019, 'Allostery in Its Many Disguises: From Theory to Applications', Structure, vol. 27, no. 4, pp. 566-578. https://doi.org/10.1016/j.str.2019.01.003
Sarker, M, Lee, HT, Mei, L, Krokhotin, A, de los Reyes, SE, Yen, L, Costantini, LM, Griffith, J, Dokholyan, NV, Alushin, GM & Campbell, SL 2019, 'Cardiomyopathy Mutations in Metavinculin Disrupt Regulation of Vinculin-Induced F-Actin Assemblies', Journal of Molecular Biology, vol. 431, no. 8, pp. 1604-1618. https://doi.org/10.1016/j.jmb.2019.02.024
Krokhotin, A, Du, H, Hirabayashi, K, Popov, K, Kurokawa, T, Wan, X, Ferrone, S, Dotti, G & Dokholyan, NV 2019, 'Computationally Guided Design of Single-Chain Variable Fragment Improves Specificity of Chimeric Antigen Receptors', Molecular Therapy - Oncolytics, vol. 15, pp. 30-37. https://doi.org/10.1016/j.omto.2019.08.008
Brodie, NI, Popov, KI, Petrotchenko, EV, Dokholyan, NV & Borchers, CH 2019, 'Conformational ensemble of native α-synuclein in solution as determined by short-distance crosslinking constraint-guided discrete molecular dynamics simulations', PLoS computational biology, vol. 15, no. 3, e1006859. https://doi.org/10.1371/journal.pcbi.1006859
Krokhotin, A, Sarker, M, Sevilla, EA, Costantini, LM, Griffith, JD, Campbell, SL & Dokholyan, NV 2019, 'Distinct Binding Modes of Vinculin Isoforms Underlie Their Functional Differences', Structure, vol. 27, no. 10, pp. 1527-1536.e3. https://doi.org/10.1016/j.str.2019.07.013
Dagliyan, O, Dokholyan, NV & Hahn, KM 2019, 'Engineering proteins for allosteric control by light or ligands', Nature Protocols, vol. 14, no. 6, pp. 1863-1883. https://doi.org/10.1038/s41596-019-0165-3
Popov, KI, Makepeace, KAT, Petrotchenko, EV, Dokholyan, NV & Borchers, CH 2019, 'Insight into the Structure of the “Unstructured” Tau Protein', Structure, vol. 27, no. 11, pp. 1710-1715.e4. https://doi.org/10.1016/j.str.2019.09.003
Sapienza, PJ, Popov, KI, Mowrey, DD, Falk, BT, Dokholyan, N & Lee, AL 2019, 'Inter-Active Site Communication Mediated by the Dimer Interface β-Sheet in the Half-the-Sites Enzyme, Thymidylate Synthase', Biochemistry, vol. 58, no. 30, pp. 3302-3313. https://doi.org/10.1021/acs.biochem.9b00486
Wang, J, Williams, B, Chirasani, VR, Krokhotin, A, Das, R & Dokholyan, NV 2019, 'Limits in accuracy and a strategy of RNA structure prediction using experimental information', Nucleic acids research, vol. 47, no. 11, pp. 5563-5572. https://doi.org/10.1093/nar/gkz427
Jiang, Y, Zhang, Y, Leung, JY, Fan, C, Popov, KI, Su, S, Qian, J, Wang, X, Holtzhausen, A, Ubil, E, Xiang, Y, Davis, I, Dokholyan, NV, Wu, G, Perou, CM, Kim, WY, Earp, HS & Liu, P 2019, 'MERTK mediated novel site Akt phosphorylation alleviates SAV1 suppression', Nature communications, vol. 10, no. 1, 1515. https://doi.org/10.1038/s41467-019-09233-7
Wang, J & Dokholyan, N 2019, 'MedusaDock 2.0: Efficient and Accurate Protein-Ligand Docking with Constraints', Journal of Chemical Information and Modeling, vol. 59, no. 6, pp. 2509-2515. https://doi.org/10.1021/acs.jcim.8b00905
Bonfanti, S, Lionetti, MC, Fumagalli, MR, Chirasani, VR, Tiana, G, Dokholyan, NV, Zapperi, S & La Porta, CAM 2019, 'Molecular mechanisms of heterogeneous oligomerization of huntingtin proteins', Scientific reports, vol. 9, no. 1, 7615. https://doi.org/10.1038/s41598-019-44151-0
Ke, W, Hong, E, Saito, RF, Rangel, MC, Wang, J, Viard, M, Richardson, M, Khisamutdinov, EF, Panigaj, M, Dokholyan, NV, Chammas, R, Dobrovolskaia, MA & Afonin, KA 2019, 'RNA-DNA fibers and polygons with controlled immunorecognition activate RNAi, FRET and transcriptional regulation of NF-κB in human cells', Nucleic acids research, vol. 47, no. 3, pp. 1350-1361. https://doi.org/10.1093/nar/gky1215
Zhu, C, Dukhovlinova, E, Council, O, Ping, L, Faison, EM, Prabhu, SS, Potter, EL, Upton, SL, Yin, G, Fay, JM, Kincer, LP, Spielvogel, E, Campbell, SL, Benhabbour, SR, Ke, H, Swanstrom, R & Dokholyan, NV 2019, 'Rationally designed carbohydrate-occluded epitopes elicit HIV-1 Env-specific antibodies', Nature communications, vol. 10, no. 1, 948. https://doi.org/10.1038/s41467-019-08876-w
Zhu, C, Han, Q, Samoshkin, A, Convertino, M, Linton, A, Faison, EM, Ji, RR, Diatchenko, L & Dokholyan, NV 2019, 'Stabilization of μ-opioid receptor facilitates its cellular translocation and signaling', Proteins: Structure, Function and Bioinformatics, vol. 87, no. 10, pp. 878-884. https://doi.org/10.1002/prot.25751
Reilley, DJ, Popov, KI, Dokholyan, NV & Alexandrova, AN 2019, 'Uncovered Dynamic Coupling Resolves the Ambiguous Mechanism of Phenylalanine Hydroxylase Oxygen Binding', Journal of Physical Chemistry B, vol. 123, no. 21, pp. 4534-4539. https://doi.org/10.1021/acs.jpcb.9b02893
Li, X, Deng, M, Petrucelli, AS, Zhu, C, Mo, J, Zhang, L, Tam, JW, Ariel, P, Zhao, B, Zhang, S, Ke, H, Li, P, Dokholyan, NV, Duncan, JA & Ting, JPY 2019, 'Viral DNA Binding to NLRC3, an Inhibitory Nucleic Acid Sensor, Unleashes STING, a Cyclic Dinucleotide Receptor that Activates Type I Interferon', Immunity, vol. 50, no. 3, pp. 591-599.e6. https://doi.org/10.1016/j.immuni.2019.02.009
Proctor, EA, Mowrey, DD & Dokholyan, NV 2019, 'β-Methylamino-L-alanine substitution of serine in SOD1 suggests a direct role in ALS etiology', PLoS computational biology, vol. 15, no. 7, e1007225. https://doi.org/10.1371/journal.pcbi.1007225

2018

Xu, L, Chirasani, VR, Carter, JS, Pasek, DA, Dokholyan, NV, Yamaguchi, N & Meissner, G 2018, 'Ca2-mediated activation of the skeletal-muscle ryanodine receptor ion channel', Journal of Biological Chemistry, vol. 293, no. 50, pp. 19501-19509. https://doi.org/10.1074/jbc.RA118.004453
Dagliyan, O, Krokhotin, A, Ozkan-Dagliyan, I, Deiters, A, Der, CJ, Hahn, KM & Dokholyan, NV 2018, 'Computational design of chemogenetic and optogenetic split proteins', Nature communications, vol. 9, no. 1, 4042. https://doi.org/10.1038/s41467-018-06531-4
Xu, L, Mowrey, DD, Chirasani, VR, Wang, Y, Pasek, DA, Dokholyan, NV & Meissner, G 2018, 'G4941K substitution in the pore-lining S6 helix of the skeletal muscle ryanodine receptor increases RyR1 sensitivity to ytosolic and luminal Ca2+', Journal of Biological Chemistry, vol. 293, no. 6, pp. 2015-2028. https://doi.org/10.1074/jbc.M117.803247
Zhang, Y, Hashemi, M, Lv, Z, Williams, B, Popov, KI, Dokholyan, NV & Lyubchenko, YL 2018, 'High-speed atomic force microscopy reveals structural dynamics of α -synuclein monomers and dimers', Journal of Chemical Physics, vol. 148, no. 12, 123322. https://doi.org/10.1063/1.5008874
Zhu, C, Beck, MV, Griffith, JD, Deshmukh, M & Dokholyan, NV 2018, 'Large SOD1 aggregates, unlike trimeric SOD1, do not impact cell viability in a model of amyotrophic lateral sclerosis', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 18, pp. 4661-4665. https://doi.org/10.1073/pnas.1800187115
Wang, C, Aleksandrov, AA, Yang, Z, Forouhar, F, Proctor, EA, Kota, P, An, J, Kaplan, A, Khazanov, N, Boël, G, Stockwell, BR, Senderowitz, H, Dokholyan, NV, Riordan, JR, Brouillette, CG & Hunt, JF 2018, 'Ligand binding to a remote site thermodynamically corrects the F508del mutation in the human cystic fibrosis transmembrane conductance regulator', Journal of Biological Chemistry, vol. 293, no. 46, pp. 17685-17704. https://doi.org/10.1074/jbc.RA117.000819
Shobair, M, Popov, KI, Dang, YL, He, H, Jackson Stutts, M & Dokholyan, NV 2018, 'Mapping allosteric linkage to channel gating by extracellular domains in the human epithelial sodium channel', Journal of Biological Chemistry, vol. 293, no. 10, pp. 3675-3684. https://doi.org/10.1074/jbc.RA117.000604
Cloer, EW, Siesser, PF, Cousins, EM, Goldfarb, D, Mowrey, DD, Harrison, JS, Weir, SJ, Dokholyan, NV & Major, MB 2018, 'P62-dependent phase separation of patient-derived KEap1 mutations and Nrf2', Molecular and cellular biology, vol. 38, no. 22, e0064417. https://doi.org/10.1128/MCB.00644-17
Rackley, L, Stewart, JM, Salotti, J, Krokhotin, A, Shah, A, Halman, JR, Juneja, R, Smollett, J, Lee, L, Roark, K, Viard, M, Tarannum, M, Vivero-Escoto, J, Johnson, PF, Dobrovolskaia, MA, Dokholyan, NV, Franco, E & Afonin, KA 2018, 'RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition', Advanced Functional Materials, vol. 28, no. 48, 1805959. https://doi.org/10.1002/adfm.201805959
Luo, J, Samanta, S, Convertino, M, Dokholyan, NV & Deiters, A 2018, 'Reversible and Tunable Photoswitching of Protein Function through Genetic Encoding of Azobenzene Amino Acids in Mammalian Cells', ChemBioChem, vol. 19, no. 20, pp. 2178-2185. https://doi.org/10.1002/cbic.201800226
Kaur, G, Guruprasad, K, Temple, BRS, Shirvanyants, DG, Dokholyan, NV & Pati, PK 2018, 'Structural complexity and functional diversity of plant NADPH oxidases', Amino Acids, vol. 50, no. 1, pp. 79-94. https://doi.org/10.1007/s00726-017-2491-5
Li, B, Tunc-Ozdemir, M, Urano, D, Jia, H, Werth, EG, Mowrey, DD, Hicks, LM, Dokholyan, NV, Torres, MP & Jones, AM 2018, 'Tyrosine phosphorylation switching of a G protein', Journal of Biological Chemistry, vol. 293, no. 13, pp. 4752-4766. https://doi.org/10.1074/jbc.RA117.000163
Han, Q, Liu, D, Convertino, M, Wang, Z, Jiang, C, Kim, YH, Luo, X, Zhang, X, Nackley, A, Dokholyan, NV & Ji, RR 2018, 'miRNA-711 Binds and Activates TRPA1 Extracellularly to Evoke Acute and Chronic Pruritus', Neuron, vol. 99, no. 3, pp. 449-463.e6. https://doi.org/10.1016/j.neuron.2018.06.039

Clinical Trials Search


Children (age < 18 years)
Adults (age >= 18 years)