Mark Hedglin, PhD - Penn State Cancer Institute
Researcher Profile
Mark Hedglin, PhD
Associate Research Professor, Chemistry
Scientific Program:Mechanisms of Carcinogenesis
Recent Publications
2025
Shin, Y, Hedglin, M & Murakami, KS 2025, 'Cryo-EM structure of apo-form human DNA polymerase δ elucidates its minimal DNA synthesis activity without PCNA', Journal of Biological Chemistry, vol. 301, no. 4, 108342. https://doi.org/10.1016/j.jbc.2025.108342
Hile, SE, Weissensteiner, MH, Pytko, KG, Dahl, J, Kejnovsky, E, Kejnovská, I, Hedglin, M, Georgakopoulos-Soares, I, Makova, KD & Eckert, KA 2025, 'Replicative DNA polymerase epsilon and delta holoenzymes show wide-ranging inhibition at G-quadruplexes in the human genome', Nucleic acids research, vol. 53, no. 8, gkaf352. https://doi.org/10.1093/nar/gkaf352
2024
Norris, JL & Hedglin, M 2024, Direct, ensemble FRET approaches to monitor transient state kinetics of human DNA polymerase δ holoenzyme assembly and initiation of DNA synthesis. in BF Eichman (ed.), Chemical and Genomic Methods in Nucleic Acid Biology. Methods in Enzymology, vol. 705, Academic Press Inc., pp. 271-309. https://doi.org/10.1016/bs.mie.2024.08.002
Muoio, D, Laspata, N, Dannenberg, RL, Curry, C, Darkoa-Larbi, S, Hedglin, M, Uttam, S & Fouquerel, E 2024, 'PARP2 promotes Break Induced Replication-mediated telomere fragility in response to replication stress', Nature communications, vol. 15, no. 1, 2857. https://doi.org/10.1038/s41467-024-47222-7
Pytko, KG, Dannenberg, RL, Eckert, KA & Hedglin, M 2024, 'Replication of [AT/TA]25 Microsatellite Sequences by Human DNA Polymerase δ Holoenzymes Is Dependent on dNTP and RPA Levels', Biochemistry, vol. 63, no. 8, pp. 969-983. https://doi.org/10.1021/acs.biochem.4c00006
Norris, JL, Rogers, LO, Pytko, KG, Dannenberg, RL, Perreault, S, Kaushik, V, Kuppa, S, Antony, E & Hedglin, M 2024, 'Replication protein A dynamically re-organizes on primer/template junctions to permit DNA polymerase δ holoenzyme assembly and initiation of DNA synthesis', Nucleic acids research, vol. 52, no. 13, pp. 7650-7664. https://doi.org/10.1093/nar/gkae475
2023
Hoitsma, NM, Norris, J, Khoang, TH, Kaushik, V, Chadda, R, Antony, E, Hedglin, M & Freudenthal, BD 2023, 'Mechanistic insight into AP-endonuclease 1 cleavage of abasic sites at stalled replication fork mimics', Nucleic acids research, vol. 51, no. 13, pp. 6738-6753. https://doi.org/10.1093/nar/gkad481
Stroik, S, Carvajal-Garcia, J, Gupta, D, Edwards, A, Luthman, A, Wyatt, DW, Dannenberg, RL, Feng, W, Kunkel, TA, Gupta, GP, Hedglin, M, Wood, R, Doublié, S, Rothenberg, E & Ramsden, DA 2023, 'Stepwise requirements for polymerases δ and θ in theta-mediated end joining', Nature, vol. 623, no. 7988, pp. 836-841. https://doi.org/10.1038/s41586-023-06729-7
2022
Dannenberg, RL, Cardina, JA, Pytko, KG & Hedglin, M 2022, 'Tracking of progressing human DNA polymerase δ holoenzymes reveals distributions of DNA lesion bypass activities', Nucleic acids research, vol. 50, no. 17, pp. 9893-9908. https://doi.org/10.1093/nar/gkac745