Researcher Profile

Researcher Profile

Susan Hafenstein, PhD

Susan Hafenstein, PhD

Associate Professor, Department of Medicine
Associate Professor, Biochemistry & Molecular Biology
Associate Professor, Department of Microbiology and Immunology
Cancer Institute, Next-Generation Therapies
Scientific Program:Mechanisms of Carcinogenesis

Research Interests

  • Capsid
  • Viruses
  • Enterovirus
  • Genome
  • Cryoelectron Microscopy
  • Parvovirus
  • Canine Parvovirus
  • Virion
  • Mutation
  • Antibodies
  • Infection
  • Transferrin Receptors

Recent Publications


Perederina, A, Li, D, Lee, H, Bator, C, Berezin, I, Hafenstein, SL & Krasilnikov, AS 2020, 'Cryo-EM structure of catalytic ribonucleoprotein complex RNase MRP', Nature communications, vol. 11, no. 1, 3474.
Voorhees, IEH, Lee, H, Allison, AB, Lopez-Astacio, R, Goodman, LB, Oyesola, OO, Omobowale, O, Fagbohun, O, Dubovi, EJ, Hafenstein, SL, Holmes, EC & Parrish, CR 2020, 'Limited intrahost diversity and background evolution accompany 40 years of canine parvovirus host adaptation and spread', Journal of virology, vol. 94, no. 1, e01162-19.
Martínez-Gualda, B, Sun, L, Martí-Marí, O, Noppen, S, Abdelnabi, R, Bator, CM, Quesada, E, Delang, L, Mirabelli, C, Lee, H, Schols, D, Neyts, J, Hafenstein, S, Camarasa, MJ, Gago, F & San-Félix, A 2020, 'Scaffold Simplification Strategy Leads to a Novel Generation of Dual Human Immunodeficiency Virus and Enterovirus-A71 Entry Inhibitors', Journal of Medicinal Chemistry, vol. 63, no. 1, pp. 349-368.


Goetschius, DJ, Parrish, CR & Hafenstein, S 2019, 'Asymmetry in icosahedral viruses', Current Opinion in Virology, vol. 36, pp. 67-73.
Goetschius, DJ, Lee, H & Hafenstein, S 2019, CryoEM reconstruction approaches to resolve asymmetric features. in FA Rey (ed.), Advances in Virus Research. Advances in Virus Research, vol. 105, Academic Press Inc., pp. 73-91.
Callaway, HM, Subramanian, S, Urbina, CA, Barnard, KN, Dick, RA, Bator, CM, Hafenstein, SL, Gifford, RJ & Parrish, CR 2019, 'Examination and reconstruction of three ancient endogenous parvovirus capsid protein gene remnants found in rodent genomes', Journal of virology, vol. 93, no. 6, e01542-18.
Subramanian, S, Maurer, AC, Bator, CM, Makhov, AM, Conway, JF, Turner, KB, Marden, JH, Vandenberghe, LH & Hafenstein, SL 2019, 'Filling Adeno-Associated Virus Capsids: Estimating Success by Cryo-Electron Microscopy', Human Gene Therapy, vol. 30, no. 12, pp. 1449-1460.
Caliaro, O, Marti, A, Ruprecht, N, Leisi, R, Subramanian, S, Hafenstein, S & Ros, C 2019, 'Parvovirus B19 uncoating occurs in the cytoplasm without capsid disassembly and it is facilitated by depletion of capsid-associated divalent cations', Viruses, vol. 11, no. 5, 430.
Lee, H, Callaway, HM, Cifuente, JO, Bator, CM, Parrish, CR & Hafenstein, SL 2019, 'Transferrin receptor binds virus capsid with dynamic motion', Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 41, pp. 20462-20471.
Sun, L, Lee, H, Thibaut, HJ, Lanko, K, Rivero-Buceta, E, Bator, C, Martinez-Gualda, B, Dallmeier, K, Delang, L, Leyssen, P, Gago, F, San-Felix, A, Hafenstein, S, Mirabelli, C & Neyts, J 2019, 'Viral engagement with host receptors blocked by a novel class of tryptophan dendrimers that targets the 5-fold-axis of the enterovirus - A 71 capsid', PLoS pathogens, vol. 15, no. 5, e1007760.


Callaway, HM, Welsch, K, Weichert, W, Allison, AB, Hafenstein, SL, Huang, K, Iketani, S & Parrish, CR 2018, 'Complex and dynamic interactions between parvovirus capsids, transferrin receptors, and antibodies control cell infection and host range', Journal of virology, vol. 92, no. 13, e00460-18.
Minns, AM, Hart, KJ, Subramanian, S, Hafenstein, S & Lindner, SE 2018, 'Nuclear, cytosolic, and surface-localized poly(A)-binding proteins of Plasmodium yoelii', mSphere, vol. 3, no. 1, e00435-17.


Subramanian, S, Organtini, LJ, Grossman, A, Domeier, PP, Cifuente, JO, Makhov, AM, Conway, JF, D'Abramo, A, Cotmore, SF, Tattersall, P & Hafenstein, S 2017, 'Cryo-EM maps reveal five-fold channel structures and their modification by gatekeeper mutations in the parvovirus minute virus of mice (MVM) capsid', Virology, vol. 510, pp. 216-223.
Guan, J, Bywaters, SM, Brendle, SA, Ashley, RE, Makhov, AM, Conway, JF, Christensen, ND & Hafenstein, S 2017, 'Cryoelectron Microscopy Maps of Human Papillomavirus 16 Reveal L2 Densities and Heparin Binding Site', Structure, vol. 25, no. 2, pp. 253-263.
Hackenbrack, N, Rogers, MB, Ashley, RE, Keel, MK, Kubiski, SV, Bryan, JA, Ghedin, E, Holmes, EC, Hafenstein, SL & Allison, AB 2017, 'Evolution and cryo-electron microscopy capsid structure of a north american bat adenovirus and its relationship to other mastadenoviruses', Journal of virology, vol. 91, no. 2, e01504-16.
Guan, J, Bywaters, SM, Brendle, SA, Ashley, RE, Makhov, AM, Conway, JF, Christensen, ND & Hafenstein, S 2017, 'High-resolution structure analysis of antibody V5 and U4 conformational epitopes on human papillomavirus 16', Viruses, vol. 9, no. 12, 374.
Organtini, LJ, Shingler, KL, Ashley, RE, Capaldi, EA, Durrani, K, Dryden, KA, Makhov, AM, Conway, JF, Pizzorno, MC & Hafenstein, S 2017, 'Honey bee deformed wing virus structures reveal that conformational changes accompany genome release', Journal of virology, vol. 91, no. 2, e01795-16.
Carson, SD, Hafenstein, S & Lee, H 2017, 'MOPS and coxsackievirus B3 stability', Virology, vol. 501, pp. 183-187.
Callaway, HM, Feng, KH, Lee, DW, Allison, AB, Pinard, M, McKenna, R, Agbandje-McKenna, M, Hafenstein, S & Parrish, CR 2017, 'Parvovirus capsid structures required for infection: Mutations controlling receptor recognition and protease cleavages', Journal of virology, vol. 91, no. 2, e01871-16.


Hegarty, JP, Sangster, W, Ashley, RE, Myers, R, Hafenstein, S & Stewart, DB 2016, Induction and purification of c. Difficile phage tail-like particles. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1476, Humana Press Inc., pp. 167-175.
Liu, X, Madhankumar, AB, Miller, PA, Duck, KA, Hafenstein, S, Rizk, E, Slagle-Webb, B, Sheehan, JM, Connor, JR & Yang, QX 2016, 'MRI contrast agent for targeting glioma: Interleukin-13 labeled liposome encapsulating gadolinium-DTPA', Neuro-oncology, vol. 18, no. 5, pp. 691-699.
Organtini, LJ, Lee, H, Iketani, S, Huang, K, Ashley, RE, Makhov, AM, Conway, JF, Parrish, CR & Hafenstein, S 2016, 'Near-atomic resolution structure of a highly neutralizing fab bound to canine parvovirus', Journal of virology, vol. 90, no. 21, pp. 9733-9742.
Allison, AB, Organtini, LJ, Zhang, S, Hafenstein, SL, Holmes, EC & Parrish, CR 2016, 'Single mutations in the VP2 300 loop region of the three-fold spike of the carnivore parvovirus capsid can determine host range', Journal of virology, vol. 90, no. 2, pp. 753-767.
Hafenstein, S 2016, 'Structural biology: A picornavirus unlike the others', Nature Microbiology, vol. 1, no. 11, 16217.
Lee, H, Shingler, KL, Organtini, LJ, Ashley, RE, Makhov, AM, Conway, JF & Hafenstein, S 2016, 'The novel asymmetric entry intermediate of a picornavirus captured with nanodiscs', Science Advances, vol. 2, no. 8, e1501929.
Thapa, V, Turner, GG, Hafenstein, S, Overton, BE, Vanderwolf, KJ & Roossinck, MJ 2016, 'Using a Novel Partitivirus in Pseudogymnoascus destructans to Understand the Epidemiology of White-Nose Syndrome', PLoS pathogens, vol. 12, no. 12, e1006076.

Clinical Trials Search

Children (age < 18 years)
Adults (age >= 18 years)