Researcher Profile

Researcher Profile

Susan Hafenstein, PhD

Susan Hafenstein, PhD

Associate Professor, Department of Medicine
Associate Professor, Biochemistry & Molecular Biology
Associate Professor, Department of Microbiology and Immunology
Scientific Program:Mechanisms of Carcinogenesis

Research Interests

  • Capsid
  • Viruses
  • Enterovirus
  • Genome
  • Cryoelectron Microscopy
  • Parvovirus
  • Virion
  • Canine Parvovirus
  • Mutation
  • Antibodies
  • Infection
  • CD55 Antigens

Recent Publications


Goetschius, DJ, Parrish, CR & Hafenstein, S 2019, 'Asymmetry in icosahedral viruses', Current Opinion in Virology, vol. 36, pp. 67-73.
Goetschius, DJ, Lee, H & Hafenstein, S 2019, CryoEM reconstruction approaches to resolve asymmetric features. in FA Rey (ed.), Advances in Virus Research. Advances in Virus Research, vol. 105, Academic Press Inc., pp. 73-91.
Callaway, HM, Subramanian, S, Urbina, CA, Barnard, KN, Dick, RA, Bator, CM, Hafenstein, S, Gifford, RJ & Parrish, CR 2019, 'Examination and reconstruction of three ancient endogenous parvovirus capsid protein gene remnants found in rodent genomes', Journal of virology, vol. 93, no. 6, e01542-18.
Caliaro, O, Marti, A, Ruprecht, N, Leisi, R, Subramanian, S, Hafenstein, S & Ros, C 2019, 'Parvovirus B19 uncoating occurs in the cytoplasm without capsid disassembly and it is facilitated by depletion of capsid-associated divalent cations', Viruses, vol. 11, no. 5, 430.
Sun, L, Lee, H, Thibaut, HJ, Lanko, K, Rivero-Buceta, E, Bator, C, Martinez-Gualda, B, Dallmeier, K, Delang, L, Leyssen, P, Gago, F, San-Félix, A, Hafenstein, S, Mirabelli, C & Neyts, J 2019, 'Viral engagement with host receptors blocked by a novel class of tryptophan dendrimers that targets the 5-fold-axis of the enterovirus-A71 capsid', PLoS pathogens, vol. 15, no. 5, pp. e1007760.


Callaway, HM, Welsch, K, Weichert, W, Allison, AB, Hafenstein, S, Huang, K, Iketani, S & Parrish, CR 2018, 'Complex and dynamic interactions between parvovirus capsids, transferrin receptors, and antibodies control cell infection and host range', Journal of virology, vol. 92, no. 13, e00460-18.
Minns, AM, Hart, KJ, Subramanian, S, Hafenstein, S & Lindner, SE 2018, 'Nuclear, cytosolic, and surface-localized poly(A)-binding proteins of Plasmodium yoelii', mSphere, vol. 3, no. 1, e00435-17.


Subramanian, S, Organtini, LJ, Grossman, A, Domeier, PP, Cifuente, JO, Makhov, AM, Conway, JF, D'Abramo, A, Cotmore, SF, Tattersall, P & Hafenstein, S 2017, 'Cryo-EM maps reveal five-fold channel structures and their modification by gatekeeper mutations in the parvovirus minute virus of mice (MVM) capsid', Virology, vol. 510, pp. 216-223.
Guan, J, Bywaters, SM, Brendle, SA, Ashley, RE, Makhov, AM, Conway, JF, Christensen, N & Hafenstein, S 2017, 'Cryoelectron Microscopy Maps of Human Papillomavirus 16 Reveal L2 Densities and Heparin Binding Site', Structure, vol. 25, no. 2, pp. 253-263.
Hackenbrack, N, Rogers, MB, Ashley, RE, Keel, MK, Kubiski, SV, Bryan, JA, Ghedin, E, Holmes, EC, Hafenstein, S & Allison, AB 2017, 'Evolution and cryo-electron microscopy capsid structure of a north american bat adenovirus and its relationship to other mastadenoviruses', Journal of virology, vol. 91, no. 2, e01504-16.
Guan, J, Bywaters, SM, Brendle, SA, Ashley, RE, Makhov, AM, Conway, JF, Christensen, N & Hafenstein, S 2017, 'High-resolution structure analysis of antibody V5 and U4 conformational epitopes on human papillomavirus 16', Viruses, vol. 9, no. 12, 374.
Organtini, LJ, Shingler, KL, Ashley, RE, Capaldi, EA, Durrani, K, Dryden, KA, Makhov, AM, Conway, JF, Pizzorno, MC & Hafenstein, S 2017, 'Honey bee deformed wing virus structures reveal that conformational changes accompany genome release', Journal of virology, vol. 91, no. 2, e01795-16.
Carson, SD, Hafenstein, S & Lee, H 2017, 'MOPS and coxsackievirus B3 stability', Virology, vol. 501, pp. 183-187.
Callaway, HM, Feng, KH, Lee, DW, Allison, AB, Pinard, M, McKenna, R, Agbandje-McKenna, M, Hafenstein, S & Parrish, CR 2017, 'Parvovirus capsid structures required for infection: Mutations controlling receptor recognition and protease cleavages', Journal of virology, vol. 91, no. 2, e01871-16.


Hegarty, JP, Sangster, W, Ashley, RE, Myers, R, Hafenstein, S & Stewart, DB 2016, Induction and purification of c. Difficile phage tail-like particles. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1476, Humana Press Inc., pp. 167-175.
Liu, X, Madhankumar, A, Miller, PA, Duck, KA, Hafenstein, S, Rizk, E, Slagle-Webb, B, Sheehan, JM, Connor, J & Yang, Q 2016, 'MRI contrast agent for targeting glioma: Interleukin-13 labeled liposome encapsulating gadolinium-DTPA', Neuro-Oncology, vol. 18, no. 5, pp. 691-699.
Organtini, LJ, Lee, H, Iketani, S, Huang, K, Ashley, RE, Makhov, AM, Conway, JF, Parrish, CR & Hafenstein, S 2016, 'Near-atomic resolution structure of a highly neutralizing fab bound to canine parvovirus', Journal of virology, vol. 90, no. 21, pp. 9733-9742.
Allison, AB, Organtini, LJ, Zhang, S, Hafenstein, S, Holmes, EC & Parrish, CR 2016, 'Single mutations in the VP2 300 loop region of the three-fold spike of the carnivore parvovirus capsid can determine host range', Journal of virology, vol. 90, no. 2, pp. 753-767.
Hafenstein, S 2016, 'Structural biology: A picornavirus unlike the others' Nature Microbiology, vol. 1, no. 11, 16217.
Lee, H, Shingler, KL, Organtini, LJ, Ashley, RE, Makhov, AM, Conway, JF & Hafenstein, S 2016, 'The novel asymmetric entry intermediate of a picornavirus captured with nanodiscs', Science Advances, vol. 2, no. 8, e1501929.
Thapa, V, Turner, GG, Hafenstein, S, Overton, BE, Vanderwolf, KJ & Roossinck, MJ 2016, 'Using a Novel Partitivirus in Pseudogymnoascus destructans to Understand the Epidemiology of White-Nose Syndrome', PLoS Pathogens, vol. 12, no. 12, e1006076.


Lee, H, Brendle, SA, Bywaters, SM, Guan, J, Ashley, RE, Yoder, JD, Makhov, AM, Conway, JF, Christensen, N & Hafenstein, S 2015, 'A cryo-electron microscopy study identifies the complete H16.V5 epitope and reveals global conformational changes initiated by binding of the neutralizing antibody fragment', Journal of virology, vol. 89, no. 2, pp. 1428-1438.
Organtini, LJ, Allison, AB, Lukk, T, Parrish, CR & Hafenstein, S 2015, 'Global displacement of canine parvovirus by a host-adapted variant: Structural comparison between pandemic viruses with distinct host ranges', Journal of virology, vol. 89, no. 3, pp. 1909-1912.
Bordería, AV, Isakov, O, Moratorio, G, Henningsson, R, Agüera-González, S, Organtini, L, Gnädig, NF, Blanc, H, Alcover, A, Hafenstein, S, Fontes, M, Shomron, N & Vignuzzi, M 2015, 'Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype', PLoS pathogens, vol. 11, no. 5, e1004838.
Pan, J, Zhang, L, Organtini, L, Hafenstein, S & Bergelson, JM 2015, 'Specificity of coxsackievirus B3 interaction with human, but not murine, decay-accelerating factor: Replacement of a single residue within short consensus repeat 2 prevents virus attachment', Journal of virology, vol. 89, no. 2, pp. 1324-1328.
Guan, J, Bywaters, SM, Brendle, SA, Lee, H, Ashley, RE, Makhov, AM, Conway, JF, Christensen, N & Hafenstein, S 2015, 'Structural comparison of four different antibodies interacting with human papillomavirus 16 and mechanisms of neutralization' Virology, vol. 483, pp. 253-263.
Nishimura, Y, McLaughlin, NP, Pan, J, Goldstein, S, Hafenstein, S, Shimizu, H, Winkler, JD & Bergelson, JM 2015, 'The Suramin Derivative NF449 Interacts with the 5-fold Vertex of the Enterovirus A71 Capsid to Prevent Virus Attachment to PSGL-1 and Heparan Sulfate', PLoS pathogens, vol. 11, no. 10, e1005184.
Guan, J, Bywaters, SM, Brendle, SA, Lee, H, Ashley, RE, Christensen, N & Hafenstein, S 2015, 'The U4 antibody epitope on human papillomavirus 16 identified by cryo-electron microscopy', Journal of virology, vol. 89, no. 23, pp. 12108-12117.
Shingler, KL, Cifuente, JO, Ashley, RE, Makhov, AM, Conway, JF & Hafenstein, S 2015, 'The enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro', Journal of virology, vol. 89, no. 3, pp. 1900-1908.

Clinical Trials Search

Children (age < 18 years)
Adults (age >= 18 years)