Researcher Profile

Researcher Profile

Susan Hafenstein, PhD

Susan Hafenstein, PhD

Associate Professor, Department of Medicine
Associate Professor, Biochemistry & Molecular Biology
Associate Professor, Department of Microbiology and Immunology
Cancer Institute, Next-Generation Therapies
Scientific Program:Mechanisms of Carcinogenesis
suh21@psu.edu

Research Interests

  • Capsid
  • Viruses
  • Enterovirus
  • Genome
  • Cryoelectron Microscopy
  • Parvovirus
  • Canine Parvovirus
  • Virion
  • Mutation
  • Antibodies
  • Infection
  • Transferrin Receptors

Recent Publications

2020

Perederina, A, Li, D, Lee, H, Bator, C, Berezin, I, Hafenstein, SL & Krasilnikov, AS 2020, 'Cryo-EM structure of catalytic ribonucleoprotein complex RNase MRP', Nature communications, vol. 11, no. 1, 3474. https://doi.org/10.1038/s41467-020-17308-z
Voorhees, IEH, Lee, H, Allison, AB, Lopez-Astacio, R, Goodman, LB, Oyesola, OO, Omobowale, O, Fagbohun, O, Dubovi, EJ, Hafenstein, SL, Holmes, EC & Parrish, CR 2020, 'Limited intrahost diversity and background evolution accompany 40 years of canine parvovirus host adaptation and spread', Journal of virology, vol. 94, no. 1, e01162-19. https://doi.org/10.1128/JVI.01162-19
Martínez-Gualda, B, Sun, L, Martí-Marí, O, Noppen, S, Abdelnabi, R, Bator, CM, Quesada, E, Delang, L, Mirabelli, C, Lee, H, Schols, D, Neyts, J, Hafenstein, S, Camarasa, MJ, Gago, F & San-Félix, A 2020, 'Scaffold Simplification Strategy Leads to a Novel Generation of Dual Human Immunodeficiency Virus and Enterovirus-A71 Entry Inhibitors', Journal of Medicinal Chemistry, vol. 63, no. 1, pp. 349-368. https://doi.org/10.1021/acs.jmedchem.9b01737

2019

Goetschius, DJ, Parrish, CR & Hafenstein, S 2019, 'Asymmetry in icosahedral viruses', Current Opinion in Virology, vol. 36, pp. 67-73. https://doi.org/10.1016/j.coviro.2019.05.006
Goetschius, DJ, Lee, H & Hafenstein, S 2019, CryoEM reconstruction approaches to resolve asymmetric features. in FA Rey (ed.), Advances in Virus Research. Advances in Virus Research, vol. 105, Academic Press Inc., pp. 73-91. https://doi.org/10.1016/bs.aivir.2019.07.007
Callaway, HM, Subramanian, S, Urbina, CA, Barnard, KN, Dick, RA, Bator, CM, Hafenstein, SL, Gifford, RJ & Parrish, CR 2019, 'Examination and reconstruction of three ancient endogenous parvovirus capsid protein gene remnants found in rodent genomes', Journal of virology, vol. 93, no. 6, e01542-18. https://doi.org/10.1128/JVI.01542-18
Subramanian, S, Maurer, AC, Bator, CM, Makhov, AM, Conway, JF, Turner, KB, Marden, JH, Vandenberghe, LH & Hafenstein, SL 2019, 'Filling Adeno-Associated Virus Capsids: Estimating Success by Cryo-Electron Microscopy', Human Gene Therapy, vol. 30, no. 12, pp. 1449-1460. https://doi.org/10.1089/hum.2019.041
Caliaro, O, Marti, A, Ruprecht, N, Leisi, R, Subramanian, S, Hafenstein, S & Ros, C 2019, 'Parvovirus B19 uncoating occurs in the cytoplasm without capsid disassembly and it is facilitated by depletion of capsid-associated divalent cations', Viruses, vol. 11, no. 5, 430. https://doi.org/10.3390/v11050430
Lee, H, Callaway, HM, Cifuente, JO, Bator, CM, Parrish, CR & Hafenstein, SL 2019, 'Transferrin receptor binds virus capsid with dynamic motion', Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 41, pp. 20462-20471. https://doi.org/10.1073/pnas.1904918116
Sun, L, Lee, H, Thibaut, HJ, Lanko, K, Rivero-Buceta, E, Bator, C, Martinez-Gualda, B, Dallmeier, K, Delang, L, Leyssen, P, Gago, F, San-Felix, A, Hafenstein, S, Mirabelli, C & Neyts, J 2019, 'Viral engagement with host receptors blocked by a novel class of tryptophan dendrimers that targets the 5-fold-axis of the enterovirus - A 71 capsid', PLoS pathogens, vol. 15, no. 5, e1007760. https://doi.org/10.1371/journal.ppat.1007760

2018

Callaway, HM, Welsch, K, Weichert, W, Allison, AB, Hafenstein, SL, Huang, K, Iketani, S & Parrish, CR 2018, 'Complex and dynamic interactions between parvovirus capsids, transferrin receptors, and antibodies control cell infection and host range', Journal of virology, vol. 92, no. 13, e00460-18. https://doi.org/10.1128/JVI.00460-18
Minns, AM, Hart, KJ, Subramanian, S, Hafenstein, S & Lindner, SE 2018, 'Nuclear, cytosolic, and surface-localized poly(A)-binding proteins of Plasmodium yoelii', mSphere, vol. 3, no. 1, e00435-17. https://doi.org/10.1128/mSphere.00435-17

2017

Subramanian, S, Organtini, LJ, Grossman, A, Domeier, PP, Cifuente, JO, Makhov, AM, Conway, JF, D'Abramo, A, Cotmore, SF, Tattersall, P & Hafenstein, S 2017, 'Cryo-EM maps reveal five-fold channel structures and their modification by gatekeeper mutations in the parvovirus minute virus of mice (MVM) capsid', Virology, vol. 510, pp. 216-223. https://doi.org/10.1016/j.virol.2017.07.015
Guan, J, Bywaters, SM, Brendle, SA, Ashley, RE, Makhov, AM, Conway, JF, Christensen, ND & Hafenstein, S 2017, 'Cryoelectron Microscopy Maps of Human Papillomavirus 16 Reveal L2 Densities and Heparin Binding Site', Structure, vol. 25, no. 2, pp. 253-263. https://doi.org/10.1016/j.str.2016.12.001
Hackenbrack, N, Rogers, MB, Ashley, RE, Keel, MK, Kubiski, SV, Bryan, JA, Ghedin, E, Holmes, EC, Hafenstein, SL & Allison, AB 2017, 'Evolution and cryo-electron microscopy capsid structure of a north american bat adenovirus and its relationship to other mastadenoviruses', Journal of virology, vol. 91, no. 2, e01504-16. https://doi.org/10.1128/JVI.01504-16
Guan, J, Bywaters, SM, Brendle, SA, Ashley, RE, Makhov, AM, Conway, JF, Christensen, ND & Hafenstein, S 2017, 'High-resolution structure analysis of antibody V5 and U4 conformational epitopes on human papillomavirus 16', Viruses, vol. 9, no. 12, 374. https://doi.org/10.3390/v9120374
Organtini, LJ, Shingler, KL, Ashley, RE, Capaldi, EA, Durrani, K, Dryden, KA, Makhov, AM, Conway, JF, Pizzorno, MC & Hafenstein, S 2017, 'Honey bee deformed wing virus structures reveal that conformational changes accompany genome release', Journal of virology, vol. 91, no. 2, e01795-16. https://doi.org/10.1128/JVI.01795-16
Carson, SD, Hafenstein, S & Lee, H 2017, 'MOPS and coxsackievirus B3 stability', Virology, vol. 501, pp. 183-187. https://doi.org/10.1016/j.virol.2016.12.002
Callaway, HM, Feng, KH, Lee, DW, Allison, AB, Pinard, M, McKenna, R, Agbandje-McKenna, M, Hafenstein, S & Parrish, CR 2017, 'Parvovirus capsid structures required for infection: Mutations controlling receptor recognition and protease cleavages', Journal of virology, vol. 91, no. 2, e01871-16. https://doi.org/10.1128/JVI.01871-16

2016

Hegarty, JP, Sangster, W, Ashley, RE, Myers, R, Hafenstein, S & Stewart, DB 2016, Induction and purification of c. Difficile phage tail-like particles. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1476, Humana Press Inc., pp. 167-175. https://doi.org/10.1007/978-1-4939-6361-4_12
Liu, X, Madhankumar, AB, Miller, PA, Duck, KA, Hafenstein, S, Rizk, E, Slagle-Webb, B, Sheehan, JM, Connor, JR & Yang, QX 2016, 'MRI contrast agent for targeting glioma: Interleukin-13 labeled liposome encapsulating gadolinium-DTPA', Neuro-oncology, vol. 18, no. 5, pp. 691-699. https://doi.org/10.1093/neuonc/nov263
Organtini, LJ, Lee, H, Iketani, S, Huang, K, Ashley, RE, Makhov, AM, Conway, JF, Parrish, CR & Hafenstein, S 2016, 'Near-atomic resolution structure of a highly neutralizing fab bound to canine parvovirus', Journal of virology, vol. 90, no. 21, pp. 9733-9742. https://doi.org/10.1128/JVI.01112-16
Allison, AB, Organtini, LJ, Zhang, S, Hafenstein, SL, Holmes, EC & Parrish, CR 2016, 'Single mutations in the VP2 300 loop region of the three-fold spike of the carnivore parvovirus capsid can determine host range', Journal of virology, vol. 90, no. 2, pp. 753-767. https://doi.org/10.1128/JVI.02636-15
Hafenstein, S 2016, 'Structural biology: A picornavirus unlike the others', Nature Microbiology, vol. 1, no. 11, 16217. https://doi.org/10.1038/nmicrobiol.2016.217
Lee, H, Shingler, KL, Organtini, LJ, Ashley, RE, Makhov, AM, Conway, JF & Hafenstein, S 2016, 'The novel asymmetric entry intermediate of a picornavirus captured with nanodiscs', Science Advances, vol. 2, no. 8, e1501929. https://doi.org/10.1126/sciadv.1501929
Thapa, V, Turner, GG, Hafenstein, S, Overton, BE, Vanderwolf, KJ & Roossinck, MJ 2016, 'Using a Novel Partitivirus in Pseudogymnoascus destructans to Understand the Epidemiology of White-Nose Syndrome', PLoS pathogens, vol. 12, no. 12, e1006076. https://doi.org/10.1371/journal.ppat.1006076

Clinical Trials Search


Children (age < 18 years)
Adults (age >= 18 years)