Researcher Profile

Researcher Profile

Susan Hafenstein, PhD

Susan Hafenstein, PhD

Professor, Biochemistry & Molecular Biology
Professor, Department of Microbiology and Immunology
Professor, Department of Medicine
Division of Infectious Diseases
Center for Infectious Disease Dynamics
Cancer Institute, Mechanisms of Carcinogenesis
Scientific Program:Next-Generation Therapies
suh21@psu.edu

Research Interests

Dr. Susan Hafenstein's research focuses on understanding the structure and functions of viruses, specifically host-virus interactions that facilitate viral entry into cells. She is an expert in cryoEM, utilizing local reconstruction approaches to solve atomic resolution maps of viruses like HPV, murine papillomavirus, polyomavirus and Zika virus.

  • Capsid
  • Viruses
  • Enterovirus
  • Genome
  • Cryoelectron Microscopy
  • Parvovirus
  • Canine Parvovirus
  • Virion
  • Mutation
  • Antibodies
  • Proteins
  • Binding Sites

Recent Publications

2022

Ye, J, Yeh, YT, Xue, Y, Wang, Z, Zhang, N, Liu, H, Zhang, K, Ricker, RA, Yu, Z, Roder, A, Lopez, NP, Organtini, L, Greene, W, Hafenstein, S, Lu, H, Ghedin, E, Terrones, M, Huang, S & Huang, SX 2022, 'Accurate virus identification with interpretable Raman signatures by machine learning', Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 23, e2118836119. https://doi.org/10.1073/pnas.2118836119

2021

Chen, YC, Dinavahi, SS, Feng, Q, Gowda, R, Ramisetti, S, Xia, X, LaPenna, KB, Chirasani, VR, Cho, SH, Hafenstein, SL, Battu, MB, Berg, A, Sharma, AK, Kirchhausen, T, Dokholyan, NV, Amin, S, He, P & Robertson, GP 2021, 'Activating Sphingosine-1-phospahte signaling in endothelial cells increases myosin light chain phosphorylation to decrease endothelial permeability thereby inhibiting cancer metastasis', Cancer Letters, vol. 506, pp. 107-119. https://doi.org/10.1016/j.canlet.2021.01.004
Hartmann, SR, Goetschius, DJ, Hu, J, Graff, JJ, Bator, CM, Christensen, ND & Hafenstein, SL 2021, 'Cryo em analysis reveals inherent flexibility of authentic murine papillomavirus capsids', Viruses, vol. 13, no. 10, 2023. https://doi.org/10.3390/v13102023
Goetschius, DJ, Hartmann, SR, Subramanian, S, Bator, CM, Christensen, ND & Hafenstein, SL 2021, 'High resolution cryo EM analysis of HPV16 identifies minor structural protein L2 and describes capsid flexibility', Scientific reports, vol. 11, no. 1, 3498. https://doi.org/10.1038/s41598-021-83076-5
Goetschius, DJ, Hartmann, SR, Organtini, LJ, Callaway, H, Huang, K, Bator, CM, Ashley, RE, Makhov, AM, Conway, JF, Parrish, CR & Hafenstein, SL 2021, 'High-resolution asymmetric structure of a Fab-virus complex reveals overlap with the receptor binding site', Proceedings of the National Academy of Sciences of the United States of America, vol. 118, no. 23, e2025452118. https://doi.org/10.1073/pnas.2025452118

2020

Lauver, MD, Goetschius, DJ, Netherby-Winslow, CS, Ayers, KN, Jin, G, Haas, DG, Frost, EL, Cho, SH, Bator, CM, Bywaters, SM, Christensen, ND, Hafenstein, SL & Lukacher, AE 2020, 'Antibody escape by polyomavirus capsid mutation facilitates neurovirulence', eLife, vol. 9, pp. 1-68. https://doi.org/10.7554/ELIFE.61056
Perederina, A, Li, D, Lee, H, Bator, C, Berezin, I, Hafenstein, SL & Krasilnikov, AS 2020, 'Cryo-EM structure of catalytic ribonucleoprotein complex RNase MRP', Nature communications, vol. 11, no. 1, 3474. https://doi.org/10.1038/s41467-020-17308-z
DiNunno, NM, Goetschius, DJ, Narayanan, A, Majowicz, SA, Moustafa, I, Bator, CM, Hafenstein, SL & Jose, J 2020, 'Identification of a pocket factor that is critical to Zika virus assembly', Nature communications, vol. 11, no. 1, 4953. https://doi.org/10.1038/s41467-020-18747-4
Voorhees, IEH, Lee, H, Allison, AB, Lopez-Astacio, R, Goodman, LB, Oyesola, OO, Omobowale, O, Fagbohun, O, Dubovi, EJ, Hafenstein, SL, Holmes, EC & Parrish, CR 2020, 'Limited intrahost diversity and background evolution accompany 40 years of canine parvovirus host adaptation and spread', Journal of virology, vol. 94, no. 1, e01162-19. https://doi.org/10.1128/JVI.01162-19
Martínez-Gualda, B, Sun, L, Martí-Marí, O, Noppen, S, Abdelnabi, R, Bator, CM, Quesada, E, Delang, L, Mirabelli, C, Lee, H, Schols, D, Neyts, J, Hafenstein, S, Camarasa, MJ, Gago, F & San-Félix, A 2020, 'Scaffold Simplification Strategy Leads to a Novel Generation of Dual Human Immunodeficiency Virus and Enterovirus-A71 Entry Inhibitors', Journal of Medicinal Chemistry, vol. 63, no. 1, pp. 349-368. https://doi.org/10.1021/acs.jmedchem.9b01737

2019

Goetschius, DJ, Parrish, CR & Hafenstein, S 2019, 'Asymmetry in icosahedral viruses', Current Opinion in Virology, vol. 36, pp. 67-73. https://doi.org/10.1016/j.coviro.2019.05.006
Goetschius, DJ, Lee, H & Hafenstein, S 2019, CryoEM reconstruction approaches to resolve asymmetric features. in FA Rey (ed.), Complementary Strategies to Understand Virus Structure and Function. Advances in Virus Research, vol. 105, Academic Press Inc., pp. 73-91. https://doi.org/10.1016/bs.aivir.2019.07.007
Callaway, HM, Subramanian, S, Urbina, CA, Barnard, KN, Dick, RA, Bator, CM, Hafenstein, SL, Gifford, RJ & Parrish, CR 2019, 'Examination and reconstruction of three ancient endogenous parvovirus capsid protein gene remnants found in rodent genomes', Journal of virology, vol. 93, no. 6, e01542-18. https://doi.org/10.1128/JVI.01542-18
Subramanian, S, Maurer, AC, Bator, CM, Makhov, AM, Conway, JF, Turner, KB, Marden, JH, Vandenberghe, LH & Hafenstein, SL 2019, 'Filling Adeno-Associated Virus Capsids: Estimating Success by Cryo-Electron Microscopy', Human Gene Therapy, vol. 30, no. 12, pp. 1449-1460. https://doi.org/10.1089/hum.2019.041
Caliaro, O, Marti, A, Ruprecht, N, Leisi, R, Subramanian, S, Hafenstein, S & Ros, C 2019, 'Parvovirus B19 uncoating occurs in the cytoplasm without capsid disassembly and it is facilitated by depletion of capsid-associated divalent cations', Viruses, vol. 11, no. 5, 430. https://doi.org/10.3390/v11050430
Lee, H, Callaway, HM, Cifuente, JO, Bator, CM, Parrish, CR & Hafenstein, SL 2019, 'Transferrin receptor binds virus capsid with dynamic motion', Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 41, pp. 20462-20471. https://doi.org/10.1073/pnas.1904918116
Sun, L, Lee, H, Thibaut, HJ, Lanko, K, Rivero-Buceta, E, Bator, C, Martinez-Gualda, B, Dallmeier, K, Delang, L, Leyssen, P, Gago, F, San-Felix, A, Hafenstein, S, Mirabelli, C & Neyts, J 2019, 'Viral engagement with host receptors blocked by a novel class of tryptophan dendrimers that targets the 5-fold-axis of the enterovirus - A 71 capsid', PLoS pathogens, vol. 15, no. 5, e1007760. https://doi.org/10.1371/journal.ppat.1007760

2018

Callaway, HM, Welsch, K, Weichert, W, Allison, AB, Hafenstein, SL, Huang, K, Iketani, S & Parrish, CR 2018, 'Complex and dynamic interactions between parvovirus capsids, transferrin receptors, and antibodies control cell infection and host range', Journal of virology, vol. 92, no. 13, e00460-18. https://doi.org/10.1128/JVI.00460-18
Minns, AM, Hart, KJ, Subramanian, S, Hafenstein, S & Lindner, SE 2018, 'Nuclear, cytosolic, and surface-localized poly(A)-binding proteins of Plasmodium yoelii', mSphere, vol. 3, no. 1, e00435-17. https://doi.org/10.1128/mSphere.00435-17

Clinical Trials Search


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Adults (age >= 18 years)