Pak Kin Wong, PhD - Penn State Cancer Institute
Researcher Profile
Pak Kin Wong, PhD
Professor, Biomedical Engineering
Professor, Department of Surgery
One Health Microbiome Center
Professor, Department of Surgery
One Health Microbiome Center
Scientific Program:Next-Generation Therapies
Recent Publications
2025
Song, Y, Soto, J, Lin, X, Hoffman, T, Hu, E, Zhu, N, Zarubova, J, Wu, Y, Tian, J, Wong, PK & Li, S 2025, 'Nano-Biosensors for mRNA-Based Cell Sorting Using Intracellular Markers at the Early Stage of Cell Reprogramming', Advanced Functional Materials, vol. 35, no. 1, 2410910. https://doi.org/10.1002/adfm.202410910
Abe, R, Lee, JH, Chin, SM, Ram-Mohan, N, Tjandra, KC, Bobenchik, AM, Mach, KE, Liao, JC, Wong, PK & Yang, S 2025, 'Precision single cell analysis to characterize host dependent antimicrobial response heterogeneity in physiological medium', Lab on a Chip, vol. 25, no. 4, pp. 714-728. https://doi.org/10.1039/d4lc00765d
2024
Lee, JH, Chin, SM, Chan, DC, Liao, JC, Yang, S, Zhang, N & Wong, PK 2024, 'Rapid Microbial Profiling through Multimodal Biosensors for Transversal Analysis', Analytical Chemistry. https://doi.org/10.1021/acs.analchem.4c02449
Li, Y, Zhu, N, Ahmed, M, Urbina, J, Huang, TY & Wong, PK 2024, 'Three-dimensional computer vision for exploring heterogeneity in collective Cancer Invasion', Scientific reports, vol. 14, no. 1, 23560. https://doi.org/10.1038/s41598-024-73688-y
Lee, JH, Chin, SM, Mach, KE, Bobenchik, AM, Liao, JC, Yang, S & Wong, PK 2024, 'Translating microbiota analysis for clinical applications', Nature Reviews Bioengineering, vol. 2, no. 4, 3347, pp. 284-286. https://doi.org/10.1038/s44222-024-00168-3
2023
Mendes, JPP, Zhu, N & Wong, PK 2023, 'A sticky-end probe biosensor for homogeneous detection of transcription factor binding activity', SLAS Technology, vol. 28, no. 5, pp. 345-350. https://doi.org/10.1016/j.slast.2023.05.001
Zhu, N, Ahmed, M, Li, Y, Liao, JC & Wong, PK 2023, 'Long noncoding RNA MALAT1 is dynamically regulated in leader cells during collective cancer invasion', Proceedings of the National Academy of Sciences of the United States of America, vol. 120, no. 27. https://doi.org/10.1073/pnas.2305410120
Li, H, Hsieh, K, Wong, PK, Mach, KE, Liao, JC & Wang, TH 2023, 'Single-cell pathogen diagnostics for combating antibiotic resistance', Nature Reviews Methods Primers, vol. 3, no. 1, 6. https://doi.org/10.1038/s43586-022-00190-y
2022
Lee, JH, van der Linden, C, Diaz, FJ & Wong, PK 2022, 'A reconfigurable microfluidic building block platform for high-throughput nonhormonal contraceptive screening', Lab on a Chip, vol. 22, no. 13, pp. 2531-2539. https://doi.org/10.1039/d2lc00424k
Chen, H, Zhu, N, Biskowitz, R, Liu, J, Deng, C, Wong, PK & Kothapalli, SR 2022, A transparent ultrasound on-chip platform enables high-throughput cell stimulation and simultaneous high-resolution optical imaging. in A Tarnok & JP Houston (eds), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX., 119640C, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11964, SPIE, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX 2022, Virtual, Online, 2/20/22. https://doi.org/10.1117/12.2610058
Meredith, CH, Castonguay, AC, Chiu, YJ, Brooks, AM, Moerman, PG, Torab, P, Wong, PK, Sen, A, Velegol, D & Zarzar, LD 2022, 'Chemical design of self-propelled Janus droplets', Matter, vol. 5, no. 2, pp. 616-633. https://doi.org/10.1016/j.matt.2021.12.014
Tjandra, KC, Ram-Mohan, N, Abe, R, Hashemi, MM, Lee, JH, Chin, SM, Roshardt, MA, Liao, JC, Wong, PK & Yang, S 2022, 'Diagnosis of Bloodstream Infections: An Evolution of Technologies towards Accurate and Rapid Identification and Antibiotic Susceptibility Testing', Antibiotics, vol. 11, no. 4, 511. https://doi.org/10.3390/antibiotics11040511
Vilchez Mercedes, SA, Bocci, F, Ahmed, M, Eder, I, Zhu, N, Levine, H, Onuchic, JN, Jolly, MK & Wong, PK 2022, 'Nrf2 Modulates the Hybrid Epithelial/Mesenchymal Phenotype and Notch Signaling During Collective Cancer Migration', Frontiers in Molecular Biosciences, vol. 9, 807324. https://doi.org/10.3389/fmolb.2022.807324
Vilchez Mercedes, SA, Eder, I, Ahmed, M, Zhu, N & Wong, PK 2022, 'Optimizing locked nucleic acid modification in double-stranded biosensors for live single cell analysis', Analyst, vol. 147, no. 4, pp. 722-733. https://doi.org/10.1039/d1an01802g
Song, Y, Soto, J, Chen, B, Hoffman, T, Zhao, W, Zhu, N, Peng, Q, Liu, L, Ly, C, Wong, PK, Wang, Y, Rowat, AC, Kurdistani, SK & Li, S 2022, 'Transient nuclear deformation primes epigenetic state and promotes cell reprogramming', Nature Materials, vol. 21, no. 10, pp. 1191-1199. https://doi.org/10.1038/s41563-022-01312-3
2021
Lee, JH, Van Der Linden, C, Diaz, FJ & Wong, PK 2021, A Reconfigurable Microfluidic Building Block Platform for High-Throughput Nonhormonal Contraceptive Screening. in Proceedings of the 15th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2021. IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED, vol. 2021-November, IEEE Computer Society, pp. 72-75, 15th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2021, Taipei, Taiwan, Province of China, 11/15/21. https://doi.org/10.1109/NANOMED54179.2021.9766590
Forsyth, B, Torab, P, Lee, JH, Malcom, T, Wang, TH, Liao, JC, Yang, S, Kvam, E, Puleo, C & Wong, PK 2021, 'A rapid single-cell antimicrobial susceptibility testing workflow for bloodstream infections', Biosensors, vol. 11, no. 8, 288. https://doi.org/10.3390/bios11080288
Chen, H, Zhu, N, Osman, M, Biskowitz, R, Liu, J, Khandare, S, Butler, P, Wong, PK & Kothapalli, SR 2021, 'A transparent low intensity pulsed ultrasound (LIPUS) chip for high-throughput cell stimulation', Lab on a Chip, vol. 21, no. 24, pp. 4734-4742. https://doi.org/10.1039/d1lc00667c
Vilchez Mercedes, SA, Bocci, F, Levine, H, Onuchic, JN, Jolly, MK & Wong, PK 2021, 'Decoding leader cells in collective cancer invasion', Nature Reviews Cancer, vol. 21, no. 9, pp. 592-604. https://doi.org/10.1038/s41568-021-00376-8
Scherer, B, Surrette, C, Li, H, Torab, P, Kvam, E, Galligan, C, Go, S, Grossmann, G, Hammond, T, Johnson, T, St-Pierre, R, Nelson, JR, Potyrailo, RA, Khire, T, Hsieh, K, Wang, TH, Wong, PK & Puleo, CM 2021, 'Digital electrical impedance analysis for single bacterium sensing and antimicrobial susceptibility testing', Lab on a Chip, vol. 21, no. 6, pp. 1073-1083. https://doi.org/10.1039/d0lc00937g
Torab, P, Yan, Y, Ahmed, M, Yamashita, H, Warrick, JI, Raman, JD, Degraff, DJ & Wong, PK 2021, 'Intratumoral heterogeneity promotes collective cancer invasion through notch1 variation', Cells, vol. 10, no. 11, 3084. https://doi.org/10.3390/cells10113084