Craig Meyers, MS, PhD
Distinguished Professor, Department of Microbiology and Immunology
Scientific Program:Mechanisms of Carcinogenesis
Presently, the central research goals of Dr. Craig Meyers' laboratory are to study the differentiation-dependent life cycle of human papillomavirus (HPV) and HPV-associated oncogenesis. Studies in the Meyers lab follow five general research themes:
- Investigation of the molecular biology and genetics of HPV morphogenesis and structure in native virus replicated under natural physiological differentiation conditions of the host tissue. The majority of the laboratories studying HPV morphogenesis and structure utilize synthetic particles such as virus-like particles (VLPs) or pseudoviral particles (PsV). The Meyers lab's studies have shown that the morphogenesis, maturation, and structure of native HPV differs in many significant characteristic from VLPs or PsV particles.
- Developing an understanding of ethnic differences in HPV types and variants using a whole-genome analysis, including infection prevalence and carcinogenicity.
- Investigating the epigenetic mechanisms of HPV-associated oral disease. This will be done by measuring genome-wide epigenetic changes occurring at different stages of the HPV16 life cycle, carcinogenic progression, and in response to ART treatment. Determining the interaction of highly active anti-retroviral drugs on oral tissues, HPV and HPV’s infection of oral tissue.
- Comparative analysis of HPV infection, life cycle and oncogenesis at different anatomical sites, including the cervix, foreskin, tonsil and anal canal.
- In addition to HPV, Dr. Meyers is also interested in studying the life cycle of other epitheliotropic infectious agents, such as the Herpes virus family, in three-dimensional human epithelial tissue.
- Life Cycle Stages
- Human papillomavirus 16
- Uterine Cervical Neoplasms
Xu, J, Liu, H, Yang, Y, Wang, X, Liu, P, Li, Y, Meyers, C, Banerjee, NS, Wang, HK, Cam, M, Lu, W, Chow, LT, Xie, X, Zhu, J & Zheng, ZM 2019, 'Genome-Wide Profiling of Cervical RNA-Binding Proteins Identifies Human Papillomavirus Regulation of RNASEH2A Expression by Viral E7 and E2F1', mBio, vol. 10, no. 1. https://doi.org/10.1128/mBio.02687-18
Kang, SD, Chatterjee, S, Alam, S, Salzberg, AC, Milici, J, Van Der Burg, SH & Meyers, C 2018, 'Effect of productive human papillomavirus 16 infection on global gene expression in cervical epithelium', Journal of virology, vol. 92, no. 20, e01261. https://doi.org/10.1128/JVI.01261-18
Israr, M, Rosenthal, D, Frejo-Navarro, L, Devoti, J, Meyers, C & Bonagura, VR 2018, 'Microarray analysis of human keratinocytes from different anatomic sites reveals site-specific immune signaling and responses to human papillomavirus type 16 transfection', Molecular Medicine, vol. 24, no. 1, 23. https://doi.org/10.1186/s10020-018-0022-9
Bywaters, SM, Brendle, SA, Biryukov, J, Wang, JW, Walston, J, Milici, J, Roden, RB, Meyers, C & Christensen, N 2018, 'Production and characterization of a novel HPV anti-L2 monoclonal antibody panel' Virology, vol. 524, pp. 106-113. https://doi.org/10.1016/j.virol.2018.08.017
Biryukov, J & Meyers, C 2018, 'Superinfection exclusion between two highrisk human papillomavirus types during a coinfection', Journal of virology, vol. 92, no. 8, e01993-17. https://doi.org/10.1128/JVI.01993-17
Chiang, C, Pauli, EK, Biryukov, J, Feister, KF, Meng, M, White, EA, Münger, K, Howley, PM, Meyers, C & Gack, MU 2018, 'The human papillomavirus E6 oncoprotein targets USP15 and TRIM25 to suppress RIG-I-mediated innate immune signaling', Journal of virology, vol. 92, no. 6, e01737-17. https://doi.org/10.1128/JVI.01737-17
Bywaters, SM, Brendle, SA, Tossi, KP, Biryukov, J, Meyers, C & Christensen, N 2017, 'Antibody competition reveals surface location of HPV L2 minor capsid protein residues 17–36', Viruses, vol. 9, no. 11, 336. https://doi.org/10.3390/v9110336
Temple, RM, Meyers, C & Sample, C 2017, Generation and infection of organotypic cultures with Epstein-Barr virus. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1532, Humana Press Inc., pp. 65-78. https://doi.org/10.1007/978-1-4939-6655-4_4
Biryukov, J, Myers, JC, McLaughlin-Drubin, ME, Griffin, HM, Milici, J, Doorbar, J & Meyers, C 2017, 'Mutations in HPV18 E1ˆE4 impact virus capsid assembly, infectivity competence, and maturation', Viruses, vol. 9, no. 12, 385. https://doi.org/10.3390/v9120385
Tang, X, Loc, WS, Dong, C, Matters, G, Butler, PJ, Kester, M, Meyers, C, Jiang, Y & Adair, JH 2017, 'The use of nanoparticulates to treat breast cancer', Nanomedicine, vol. 12, no. 19, pp. 2367-2388. https://doi.org/10.2217/nnm-2017-0202
Meyers, C, Milici, J & Robison, R 2017, 'UVC radiation as an effective disinfectant method to inactivate human papillomaviruses', PloS one, vol. 12, no. 10, e0187377. https://doi.org/10.1371/journal.pone.0187377
Wang, X, Liu, H, Ge, H, Ajiro, M, Sharma, NR, Meyers, C, Morozov, P, Tuschl, T, Klar, A, Court, D & Zhenga, ZM 2017, 'Viral DNA replication orientation and hnrnps regulate transcription of the human papillomavirus 18 late promoter', mBio, vol. 8, no. 3, e00713-17. https://doi.org/10.1128/mBio.00713-17
Sharma, NR, Wang, X, Majerciak, V, Ajiro, M, Kruhlak, M, Meyers, C & Zheng, ZM 2016, 'Cell type- and tissue context-dependent nuclear distribution of human Ago2', Journal of Biological Chemistry, vol. 291, no. 5, pp. 2302-2309. https://doi.org/10.1074/jbc.C115.695049
Israr, M, Biryukov, J, Ryndock, EJ, Alam, S & Meyers, C 2016, 'Comparison of human papillomavirus type 16 replication in tonsil and foreskin epithelia', Virology, vol. 499, pp. 82-90. https://doi.org/10.1016/j.virol.2016.09.004
Wang, X, Liu, H, Wang, HK, Meyers, C, Chow, L & Zheng, ZM 2016, 'HPV18 DNA replication inactivates the early promoter P55 activity and prevents viral E6 expression', Virologica Sinica, vol. 31, no. 5, pp. 437-440. https://doi.org/10.1007/s12250-016-3887-1
Ma, W, Tummers, B, van Esch, EMG, Goedemans, R, Melief, CJM, Meyers, C, Boer, JM & van der Burg, SH 2016, 'Human papillomavirus downregulates the expression of IFITM1 and RIPK3 to escape from IFNγ- and TNFα-mediated antiproliferative effects and necroptosis', Frontiers in immunology, vol. 7, no. NOV, 496. https://doi.org/10.3389/fimmu.2016.00496
Ryndock, E, Robison, R & Meyers, C 2016, 'Susceptibility of HPV16 and 18 to high level disinfectants indicated for semi-critical ultrasound probes', Journal of Medical Virology, vol. 88, no. 6, pp. 1076-1080. https://doi.org/10.1002/jmv.24421
Cruz, L, Biryukov, J, Conway, MJ & Meyers, C 2015, 'Cleavage of the HPV16 minor capsid protein L2 during virion morphogenesis ablates the requirement for cellular furin during de novo infection', Viruses, vol. 7, no. 11, pp. 5813-5830. https://doi.org/10.3390/v7112910
Du, P, Camacho, F, McCall-Hosenfeld, J, Lengerich, E, Meyers, C & Christensen, N 2015, 'Human papillomavirus vaccination among adults and children in 5 US states', Journal of Public Health Management and Practice, vol. 21, no. 6, pp. 573-583. https://doi.org/10.1097/PHH.0000000000000271
Biryukov, J & Meyers, C 2015, 'Papillomavirus infectious pathways: A comparison of systems', Viruses, vol. 7, no. 8, pp. 4303-4325. https://doi.org/10.3390/v7082823
Ryndock, EJ, Biryukov, J & Meyers, C 2015, 'Replication of human papillomavirus in culture' Methods in Molecular Biology, vol. 1249, pp. 39-52. https://doi.org/10.1007/978-1-4939-2013-6_3
Tummers, B, Goedemans, R, Pelascini, LPL, Jordanova, ES, Van Esch, EMG, Meyers, C, Melief, CJM, Boer, JM & Van Der Burg, SH 2015, 'The interferon-related developmental regulator 1 is used by human papillomavirus to suppress NFκB activation', Nature communications, vol. 6, 6537. https://doi.org/10.1038/ncomms7537