Che-Yen "Joseph" Wang, PhD - Penn State Cancer Institute
Researcher Profile
Che-Yen "Joseph" Wang, PhD
Assistant Professor, Department of Microbiology and Immunology
Scientific Program:Mechanisms of Carcinogenesis
Research Interests
The overall focus of Dr. Joseph Wang's laboratory is to understand the molecular mechanisms underlying the complex life cycle of viruses using cryo-electron (cryo-EM) microscopy. His laboratory utilizes both single particle and electron tomography approaches to study virus structures as well as the interactions between viral proteins and host factors. The optimal goal is to use obtained structural information for developing effective drug intervention against virus infection.
Currently, Dr. Wang's lab has three major focuses:
- Structural study of Hepatitis B virus (HBV) and subviral particles
- Dissecting the molecular details of HBV entry and fusion process
- Molecular mechanism of alphavirus fusion
- Capsid
- Hepatitis B virus
- Virion
- Proteins
- Viruses
- Capsid Proteins
- RNA
- Viral Core Proteins
- Virus Assembly
- In Vitro Techniques
- Alphavirus
- DNA
Recent Publications
2023
Hong, X, Bianchini, EN, Wang, JCY & Hu, J 2023, 'Characterization of Intracellular Precore-Derived Proteins and Their Functions in Hepatitis B Virus-Infected Human Hepatocytes', mBio, vol. 14, no. 1. https://doi.org/10.1128/mbio.03501-22
DiNunno, N, Bianchini, EN, Liu, H & Wang, JCY 2023, 'Protein Structure Predictions, Atomic Model Building, and Validation Using a Cryo-EM Density Map from Hepatitis B Virus Spherical Subviral Particle', Bio-protocol, vol. 13, no. 14, e4751. https://doi.org/10.21769/BioProtoc.4751
Kim, C, Schlicksup, CJ, Pérez-Segura, C, Hadden-Perilla, JA, Wang, JCY & Zlotnick, A 2023, 'Structure of the Hepatitis B virus capsid quasi-6-fold with a trapped C-terminal domain reveals capsid movements associated with domain exit', Journal of Biological Chemistry, vol. 299, no. 9, 105104. https://doi.org/10.1016/j.jbc.2023.105104
2022
Kim, C, Barnes, LF, Schlicksup, CJ, Patterson, AJ, Bothner, B, Jarrold, MF, Wang, CYJ & Zlotnick, A 2022, 'Core Protein-Directed Antivirals and Importin b Can Synergistically Disrupt Hepatitis B Virus Capsids', Journal of virology, vol. 96, no. 2, A4. https://doi.org/10.1128/JVI.01395-21
Liu, H, Hong, X, Xi, J, Menne, S, Hu, J & Che-Yen Wang, J 2022, 'Cryo-EM structures of human hepatitis B and woodchuck hepatitis virus small spherical subviral particles', Science Advances, vol. 8, no. 31, eabo4184. https://doi.org/10.1126/sciadv.abo4184
Robins, LI, Clark, A, Gafken, PR, Alam, S, Milici, J, Hassan, R, Wang, CY, Williams, J & Meyers, C 2022, 'Hypochlorous acid as a disinfectant for high-risk HPV: Insight into the mechanism of action', Journal of Medical Virology, vol. 94, no. 7, pp. 3386-3393. https://doi.org/10.1002/jmv.27716
Ren, SC, Qazi, SA, Towell, B, Wang, JCY & Mukhopadhyay, S 2022, 'Mutations at the Alphavirus E1'-E2 Interdimer Interface Have Host-Specific Phenotypes', Journal of virology, vol. 96, no. 5, e02149-21. https://doi.org/10.1128/jvi.02149-21
Xi, J, Cui, X, Liu, K, Liu, H, Wang, J & Hu, J 2022, 'Region-Specific Hepatitis B Virus Genome Exposure from Nucleocapsid Modulated by Capsid Linker Sequence and Inhibitor: Implications for Uncoating', Journal of virology, vol. 96, no. 8. https://doi.org/10.1128/jvi.00399-22
2021
Zhao, Z, Wang, JCY, Zhang, M, Lyktey, NA, Jarrold, MF, Jacobson, SC & Zlotnick, A 2021, 'Asymmetrizing an icosahedral virus capsid by hierarchical assembly of subunits with designed asymmetry', Nature communications, vol. 12, no. 1, 589. https://doi.org/10.1038/s41467-020-20862-1
Goodall, CP, Schwarz, B, Selivanovitch, E, Avera, J, Wang, J, Miettinen, H & Douglas, T 2021, 'Controlled Modular Multivalent Presentation of the CD40 Ligand on P22 Virus-like Particles Leads to Tunable Amplification of CD40 Signaling', ACS Applied Bio Materials, vol. 4, no. 12, pp. 8205-8214. https://doi.org/10.1021/acsabm.1c00718
Wang, JCY & Chen, L 2021, 'Publisher Correction: Structural basis for the structural dynamics of human mitochondrial chaperonin mHsp60 (Scientific Reports, (2021), 11, 1, (14809), 10.1038/s41598-021-94236-y)', Scientific reports, vol. 11, no. 1, 17372. https://doi.org/10.1038/s41598-021-96945-w
Truong, CD, Williams, DR, Zhu, M, Wang, JCY & Chiu, PL 2021, 'Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies', Journal of Visualized Experiments, vol. 2021, no. 177, e63015. https://doi.org/10.3791/63015
Wang, JCY & Chen, L 2021, 'Structural basis for the structural dynamics of human mitochondrial chaperonin mHsp60', Scientific reports, vol. 11, no. 1, 14809. https://doi.org/10.1038/s41598-021-94236-y
Fukudome, A, Singh, J, Mishra, V, Reddem, E, Martinez-Marquez, F, Wenzel, S, Yan, R, Shiozaki, M, Yu, Z, Wang, JCY, Takagi, Y & Pikaard, CS 2021, 'Structure and RNA template requirements of Arabidopsis RNA-DEPENDENT RNA POLYMERASE 2', Proceedings of the National Academy of Sciences of the United States of America, vol. 118, no. 51, e2115899118. https://doi.org/10.1073/pnas.2115899118
2020
Zhou, W, Tahir, F, Wang, JCY, Woodson, M, Sherman, MB, Karim, S, Neelakanta, G & Sultana, H 2020, 'Discovery of Exosomes From Tick Saliva and Salivary Glands Reveals Therapeutic Roles for CXCL12 and IL-8 in Wound Healing at the Tick–Human Skin Interface', Frontiers in Cell and Developmental Biology, vol. 8, 554. https://doi.org/10.3389/fcell.2020.00554
Schlicksup, CJ, Laughlin, P, Dunkelbarger, S, Wang, JCY & Zlotnick, A 2020, 'Local Stabilization of Subunit-Subunit Contacts Causes Global Destabilization of Hepatitis B Virus Capsids', ACS chemical biology, vol. 15, no. 6, pp. 1708-1717. https://doi.org/10.1021/acschembio.0c00320
Button, JM, Qazi, SA, Wang, JCY & Mukhopadhyay, S 2020, 'Revisiting an old friend: new findings in alphavirus structure and assembly', Current Opinion in Virology, vol. 45, pp. 25-33. https://doi.org/10.1016/j.coviro.2020.06.005
Hadden-Perilla, JA, Zlotnick, A, Zhao, Z, Wang, JCY & Segura, CP 2020, 'The integrity of the intradimer interface of the hepatitis B virus capsid protein dimer regulates capsid self-assembly', ACS chemical biology, vol. 15, no. 12, pp. 3124-3132. https://doi.org/10.1021/acschembio.0c00277
Bond, K, Tsvetkova, IB, Wang, JCY, Jarrold, MF & Dragnea, B 2020, 'Virus Assembly Pathways: Straying Away but Not Too Far', Small, vol. 16, no. 51, 2004475. https://doi.org/10.1002/smll.202004475