Xiaojun Lian - Penn State Cancer Institute
Researcher Profile
Xiaojun Lian
Associate Professor, Biomedical Engineering
Center for Neural Engineering
Center for Neural Engineering
Scientific Program:Mechanisms of Carcinogenesis
Research Interests
- Pluripotent Stem Cells
- Cardiac Myocytes
- Clustered Regularly Interspaced Short Palindromic Repeats
- Heart
- Catenins
- Gene Editing
- Stem Cells
- Human Embryonic Stem Cells
- Cell Differentiation
- Endothelial Cells
- Intercellular Signaling Peptides and Proteins
- Human Development
Recent Publications
2024
Ream, MW, Randolph, LN, Jiang, Y, Chang, Y, Bao, X & Lian, XL 2024, 'Direct programming of human pluripotent stem cells into endothelial progenitors with SOX17 and FGF2', Stem Cell Reports, vol. 19, no. 4, pp. 579-595. https://doi.org/10.1016/j.stemcr.2024.02.006
Haideri, T, Lin, J, Bao, X & Lian, XL 2024, 'MAGIK: A rapid and efficient method to create lineage-specific reporters in human pluripotent stem cells', Stem Cell Reports, vol. 19, no. 5, pp. 744-757. https://doi.org/10.1016/j.stemcr.2024.03.005
Seitz, MP, Song, Y, Lian, XL, Ma, Z & Jain, E 2024, 'Soft Polyethylene Glycol Hydrogels Support Human PSC Pluripotency and Morphogenesis', ACS Biomaterials Science and Engineering, vol. 10, no. 7, pp. 4525-4540. https://doi.org/10.1021/acsbiomaterials.4c00923
2023
Howells, AR, Welch, PJ, Kim, J, Forest, CR, Shi, C & Lian, XL 2024, 'A drug-selectable acoustic reporter gene system for human cell ultrasound imaging', Bioengineering and Translational Medicine, vol. 9, no. 2, e10584. https://doi.org/10.1002/btm2.10584
Chang, Y, Cai, X, Syahirah, R, Yao, Y, Xu, Y, Jin, G, Bhute, VJ, Torregrosa-Allen, S, Elzey, BD, Won, YY, Deng, Q, Lian, XL, Wang, X, Eniola-Adefeso, O & Bao, X 2023, 'CAR-neutrophil mediated delivery of tumor-microenvironment responsive nanodrugs for glioblastoma chemo-immunotherapy', Nature communications, vol. 14, no. 1, 2266. https://doi.org/10.1038/s41467-023-37872-4
Jung, J, Chang, Y, Jin, G, Deng, Q, Lian, XL & Bao, X 2023, 'Chemically defined generation of human definitive hematopoietic stem and progenitor cells', STAR Protocols, vol. 4, no. 1, 101953. https://doi.org/10.1016/j.xpro.2022.101953
Wang, K, Liu, X, Lian, XL, Bao, X & Li, K 2023, 'Editorial: Bioengineered gene and cell therapy for treating cardiovascular diseases', Frontiers in Bioengineering and Biotechnology, vol. 11, 1250175. https://doi.org/10.3389/fbioe.2023.1250175
Chang, Y, Hummel, SN, Watson, MN, Jin, G, Lian, XL & Bao, X 2023, 'Engineered Artificial Human Neutrophils Exhibit Mature Functional Performance', ACS Synthetic Biology, vol. 12, no. 8, pp. 2262-2270. https://doi.org/10.1021/acssynbio.3c00309
Chang, Y, Jin, G, Luo, W, Luo, Q, Jung, J, Hummel, SN, Torregrosa-Allen, S, Elzey, BD, Low, PS, Lian, XL & Bao, X 2023, 'Engineered human pluripotent stem cell-derived natural killer cells with PD-L1 responsive immunological memory for enhanced immunotherapeutic efficacy', Bioactive Materials, vol. 27, pp. 168-180. https://doi.org/10.1016/j.bioactmat.2023.03.018
Wang, O, Han, L, Lin, H, Tian, M, Zhang, S, Duan, B, Chung, S, Zhang, C, Lian, X, Wang, Y & Lei, Y 2023, 'Fabricating 3-dimensional human brown adipose microtissues for transplantation studies', Bioactive Materials, vol. 22, pp. 518-534. https://doi.org/10.1016/j.bioactmat.2022.10.022
Nouri, R, Jiang, Y, Politza, AJ, Liu, T, Greene, WH, Zhu, Y, Nunez, JJ, Lian, X & Guan, W 2023, 'STAMP-Based Digital CRISPR-Cas13a for Amplification-Free Quantification of HIV-1 Plasma Viral Loads', ACS nano, vol. 17, no. 11, pp. 10701-10712. https://doi.org/10.1021/acsnano.3c01917
2022
Chang, Y, Syahirah, R, Oprescu, SN, Wang, X, Jung, J, Cooper, SH, Torregrosa-Allen, S, Elzey, BD, Hsu, AY, Randolph, LN, Sun, Y, Kuang, S, Broxmeyer, HE, Deng, Q, Lian, X & Bao, X 2022, 'Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells', Biomaterials, vol. 285, 121569. https://doi.org/10.1016/j.biomaterials.2022.121569
Nouri, R, Jiang, Y, Politza, AJ, Lian, XL & Guan, W 2022, Digital CRISPR-based quantification of HIV-1. in 2022 IEEE Sensors, SENSORS 2022 - Conference Proceedings. Proceedings of IEEE Sensors, vol. 2022-October, Institute of Electrical and Electronics Engineers Inc., 2022 IEEE Sensors Conference, SENSORS 2022, Dallas, United States, 10/30/22. https://doi.org/10.1109/SENSORS52175.2022.9967104
Chang, Y, Syahirah, R, Wang, X, Jin, G, Torregrosa-Allen, S, Elzey, BD, Hummel, SN, Wang, T, Li, C, Lian, X, Deng, Q, Broxmeyer, HE & Bao, X 2022, 'Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy', Cell Reports, vol. 40, no. 3, 111128. https://doi.org/10.1016/j.celrep.2022.111128
Jiang, Y, Hoenisch, RC, Chang, Y, Bao, X, Cameron, CE & Lian, XL 2022, 'Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems', Bioactive Materials, vol. 14, pp. 313-320. https://doi.org/10.1016/j.bioactmat.2022.01.046
Haideri, T, Howells, A, Jiang, Y, Yang, J, Bao, X & Lian, XL 2022, 'Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells', Cell Reports Methods, vol. 2, no. 9, 100290. https://doi.org/10.1016/j.crmeth.2022.100290
Jung, J, Chang, Y, Jin, G, Lian, X & Bao, X 2022, 'Temporal Expression of Transcription Factor ID2 Improves Natural Killer Cell Differentiation from Human Pluripotent Stem Cells', ACS Synthetic Biology, vol. 11, no. 6, pp. 2001-2008. https://doi.org/10.1021/acssynbio.2c00017
Liang, PY, Chang, Y, Jin, G, Lian, X & Bao, X 2022, 'Wnt signaling directs human pluripotent stem cells into vascularized cardiac organoids with chamber-like structures', Frontiers in Bioengineering and Biotechnology, vol. 10, 1059243. https://doi.org/10.3389/fbioe.2022.1059243
2021
Jiang, Y, Bao, X & Lian, XL 2021, 'A dual cardiomyocyte reporter model derived from human pluripotent stem cells', Stem Cell Research and Therapy, vol. 12, no. 1, 305. https://doi.org/10.1186/s13287-021-02341-6
Nouri, R, Jiang, Y, Tang, Z, Lian, XL & Guan, W 2021, 'Detection of SARS-CoV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores', Nano letters, vol. 21, no. 19, pp. 8393-8400. https://doi.org/10.1021/acs.nanolett.1c02974
Jiang, Y, Chen, C, Randolph, LN, Ye, S, Zhang, X, Bao, X & Lian, XL 2021, 'Generation of pancreatic progenitors from human pluripotent stem cells by small molecules', Stem Cell Reports, vol. 16, no. 9, pp. 2395-2409. https://doi.org/10.1016/j.stemcr.2021.07.021
Zhu, J, Liu, S, Hu, Z, Zhang, X, Yi, N, Tang, K, Dexheimer, MG, Lian, X, Wang, Q, Yang, J, Gray, J & Cheng, H 2021, 'Laser-induced graphene non-enzymatic glucose sensors for on-body measurements', Biosensors and Bioelectronics, vol. 193, 113606. https://doi.org/10.1016/j.bios.2021.113606
Hellwarth, PB, Chang, Y, Das, A, Liang, PY, Lian, X, Repina, NA & Bao, X 2021, 'Optogenetic-mediated cardiovascular differentiation and patterning of human pluripotent stem cells', Advanced Genetics, vol. 2, no. 3, e202100011. https://doi.org/10.1002/ggn2.202100011
Nouri, R, Jiang, Y, Tang, Z, Lian, XL & Guan, W 2021, Sequence-Specific Recognition of SARS COV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN). in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 833-834, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21.
2020
Zhao, N, Coyne, J, Abune, L, Shi, P, Lian, XL, Zhang, G & Wang, Y 2020, 'Exogenous Signaling Molecules Released from Aptamer-Functionalized Hydrogels Promote the Survival of Mesenchymal Stem Cell Spheroids', ACS Applied Materials and Interfaces, vol. 12, no. 22, pp. 24599-24610. https://doi.org/10.1021/acsami.0c05681
Chang, Y, Hellwarth, PB, Randolph, LN, Sun, Y, Xing, Y, Zhu, W, Lian, XL & Bao, X 2020, 'Fluorescent indicators for continuous and lineage-specific reporting of cell-cycle phases in human pluripotent stem cells', Biotechnology and bioengineering, vol. 117, no. 7, pp. 2177-2186. https://doi.org/10.1002/bit.27352
Jiang, Y & Lian, XL 2020, 'Heart regeneration with human pluripotent stem cells: Prospects and challenges', Bioactive Materials, vol. 5, no. 1, pp. 74-81. https://doi.org/10.1016/j.bioactmat.2020.01.003
Kim, GB, Aragon-Sanabria, V, Randolph, L, Jiang, H, Reynolds, JA, Webb, BS, Madhankumar, A, Lian, X, Connor, JR, Yang, J & Dong, C 2020, 'High-affinity mutant Interleukin-13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma', Bioactive Materials, vol. 5, no. 3, pp. 624-635. https://doi.org/10.1016/j.bioactmat.2020.04.011
Nouri, R, Jiang, Y, Lian, XL & Guan, W 2020, 'Sequence-Specific Recognition of HIV-1 DNA with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)', ACS Sensors, vol. 5, no. 5, pp. 1273-1280. https://doi.org/10.1021/acssensors.0c00497